Avaliação do efeito antibiofilme de um enxaguatório bucal contendo extrato da casca de romã (Punica granatum), trimetafosfato de sódio e flúor.
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Data
2020-11-27
Autores
Curti, Walter Ariel [UNESP]
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Universidade Estadual Paulista (Unesp)
Resumo
O presente estudo avaliou a ação de formulações não alcoólicas para enxaguatório bucal contendo extrato da casca da romã (Punica granatum) (E), trimetafosfato de sódio (T) e flúor (F) contra biofilmes formados por Candida albicans e Streptococcus mutans, e sua ação sobre o pH destes biofilmes. O extrato foi obtido da casca desidratada da romã (E) por meio do processo de filtração e maceração, sendo utilizado a uma concentração de 3% nas formulações. Já as concentrações de T e F foram de 0,3% e 225 ppm nas formulações. Os grupos de formulações avaliados foram: - ETF (contendo todos os ativos), TF (contendo somente T e F) e E (formulação somente com extrato da casca de romã). Como controles utilizou-se Listerine® (C+) também livre de álcool e com concentração de flúor de 220 ppm, e solução salina (C-). Os biofilmes duplos de Candida albicans (ATCC 10231) e Streptococcus mutans (ATCC 25175) foram crescidos por 24 horas sobre a superfície de discos de hidroxiapatia previamente tratados por 1 minuto com cada uma das formulações. Os biofilmes de 24 horas foram tratados novamente por 1 ou 10 minutos após a sua formação, e as células viáveis dos biofilmes foram quantificadas. Os dados foram submetidos ao teste de normalidade (Shapiro-Wilk), seguido de ANOVA de um fator e o teste de comparação múltipla de Holm-Sidak, todos com nível de significância de 1%. Todas as formulações reduziram estatisticamente (p<0,01) o biofilme de C. albicans e S. mutans em relação ao controle negativo, e as maiores reduções ocorreram com o Listerine, independentemente do tempo de tratamento realizado. Para C. albicans as maiores reduções no número de células viáveis (p<0,01) ocorreram com o tratamento de 10 minutos. Já para o biofilme de S. mutans, o tempo de tratamento de 10 minutos apresentou-se mais efetivo (p<0,05) somente nos tratamentos com as formulações ETF e TF. Ainda, o tratamento de 1 ou 10 minutos não interferiu no pH dos biofilmes de todos os grupos avaliados. Conclui-se que, embora o pH dos biofilmes tenha se apresentado mais ácido para as formulações testadas comparativamente aos controles, as formulações para enxaguatório bucal testadas apresentaram expressiva atividade antibiofilme contra microrganismos importantes relacionados à cárie dental.
The present study evaluated the action of non-alcoholic mouthwash formulations containing pomegranate peel extract (Punica granatum) (E), sodium trimetaphosphate (T) and fluorine (F) against biofilms formed by Candida albicans and Streptococcus mutans, and their action on the pH of these biofilms. The extract was obtained from the dehydrated pomegranate peel (E) through the filtration and maceration process, being used at a concentration of 3% in the formulations. The concentrations of T and F were 0.3% and 225 ppm in the formulations. The groups of formulations evaluated were: - ETF (containing all assets), TF (containing only T and F) and E (formulation with extract of pomegranate peel only). As controls, Listerine® (C +) was also used, free of alcohol and with a fluoride concentration of 220 ppm, and saline solution (C-). The double biofilms of Candida albicans (ATCC 10231) and Streptococcus mutans (ATCC 25175) were grown for 24 hours on the surface of hydroxyapathy discs previously treated for 1 minute with each of the formulations. The 24-hour biofilms were treated again for 1 or 10 minutes after their formation, and the viable biofilm cells were quantified. The data were submitted to the normality test (Shapiro-Wilk), followed by one-way ANOVA and the Holm-Sidak multiple comparison test, all with a 1% significance level. All formulations reduced statistically (p <0.01) the biofilm of C. albicans and S. mutans in relation to the negative control, and the greatest reductions occurred with Listerine, regardless of the treatment time performed. For C. albicans, the greatest reductions in the number of viable cells (p <0.01) occurred with the 10-minute treatment. For the S. mutans biofilm, the 10-minute treatment time was more effective (p <0.05) only for treatments with ETF and TF formulations. Still, the treatment of 1 or 10 minutes did not affect the pH of the biofilms of all the groups evaluated. It is concluded that, although the pH of biofilms was more acidic for the tested formulations compared to controls, the tested mouthwash formulations showed significant antibiofilm activity against important microorganisms related to dental caries.
The present study evaluated the action of non-alcoholic mouthwash formulations containing pomegranate peel extract (Punica granatum) (E), sodium trimetaphosphate (T) and fluorine (F) against biofilms formed by Candida albicans and Streptococcus mutans, and their action on the pH of these biofilms. The extract was obtained from the dehydrated pomegranate peel (E) through the filtration and maceration process, being used at a concentration of 3% in the formulations. The concentrations of T and F were 0.3% and 225 ppm in the formulations. The groups of formulations evaluated were: - ETF (containing all assets), TF (containing only T and F) and E (formulation with extract of pomegranate peel only). As controls, Listerine® (C +) was also used, free of alcohol and with a fluoride concentration of 220 ppm, and saline solution (C-). The double biofilms of Candida albicans (ATCC 10231) and Streptococcus mutans (ATCC 25175) were grown for 24 hours on the surface of hydroxyapathy discs previously treated for 1 minute with each of the formulations. The 24-hour biofilms were treated again for 1 or 10 minutes after their formation, and the viable biofilm cells were quantified. The data were submitted to the normality test (Shapiro-Wilk), followed by one-way ANOVA and the Holm-Sidak multiple comparison test, all with a 1% significance level. All formulations reduced statistically (p <0.01) the biofilm of C. albicans and S. mutans in relation to the negative control, and the greatest reductions occurred with Listerine, regardless of the treatment time performed. For C. albicans, the greatest reductions in the number of viable cells (p <0.01) occurred with the 10-minute treatment. For the S. mutans biofilm, the 10-minute treatment time was more effective (p <0.05) only for treatments with ETF and TF formulations. Still, the treatment of 1 or 10 minutes did not affect the pH of the biofilms of all the groups evaluated. It is concluded that, although the pH of biofilms was more acidic for the tested formulations compared to controls, the tested mouthwash formulations showed significant antibiofilm activity against important microorganisms related to dental caries.
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Palavras-chave
Punicaceae, Polifosfatos, Flúor, Biofilmes, Polyphosphates