Osteogênese periimplantar em função da ozonioterapia na osteoporose induzida em ratas
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Data
2022-04-27
Autores
Souza, Mônica Caroline de [UNESP]
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Universidade Estadual Paulista (Unesp)
Resumo
Devido às suas propriedades, a ozonioterapia surge como um método interessante para a análise do reparo ósseo periimplantar, principalmente em ossos de baixa densidade, onde procura-se diminuir a taxa de insucesso dos implantes ósseo-integráveis. O objetivo do presente trabalho foi analisar o efeito da ozonioterapia no reparo ósseo periimplantar em ratas submetidas à indução de osteoporose. Para tal, foram utilizadas 10 ratas, adultas, com 6 meses de idade. Após o procedimento de ovariectomia bilateral e uma espera de 90 dias para a indução da osteoporose, foi realizada a cirurgia para instalação dos implantes, sendo que cada animal teve 1 implante instalado em uma de suas tíbias. O tratamento teste (OVX OZ) foi realizado com a aplicação intraperitoneal do ozônio em concentração de 0.7mg/kg, nos períodos: PO imediato, 2º, 4º, 6º, 8º, 10º, 12º dia pós-operatório. O controle (OVX SAL) foi realizado a aplicação intraperitoneal de 1ml de solução salina, nos mesmos períodos. Os tempos de eutanásia foram aos 14 e 42 dias para análises histométrica e imunohistoquímica. Ao analisar a extensão linear de contato osso e implante (ELCOI) e área de osso neoformado (AON), houve significância estatística apenas aos 14 dias (p<0,05) com maiores valores para OVX OZ. Na comparação intragrupo OVX OZ, entre 14 e 42 dias, não houve significância estatística. Já na comparação intragrupo SAL, entre 14 e 42 dias, houve diferença estatística significante, tanto para ELCOI como para AON. O anticorpo contra osteocalcina, no período de 14 dias apresentou score 2 (marcação moderada) para o grupo OVX OZ, e score 1 (leve) para o grupo OVX SAL. Aos 42 dias apresentou marcação moderada para os grupos OVX OZ e OVX SAL. A ozonioterapia modifica positivamente o mecanismo biológico de reparo nos períodos iniciais, através da liberação de fatores de crescimento no citoplasma. Alguns são osteoindutores, agindo nas células osteoprogenitoras, diferenciando-as e auxiliando na osteogênese. Dessa forma, concluiu-se que, a ozonioterapia favoreceu o reparo ósseo periimplantar nos estágios iniciais.
Due to its properties, ozone therapy appears as an interesting method for the analysis of peri-implant bone repair, especially in low-density bones, where the aim is to reduce the rate of bone-integrating implants. objective of the present study analyzed the effect of therapy on peri-implant bone repair in peri-implant rats the effect of inducing osteoporosis. For this, 10 rats, adults, with 6 months of age were used. After the bilateral ovariectomy procedure and a 90-day wait for the induction of osteoporosis, a surgery was performed to install the implants, and each animal had 1 implant in one of its installed tibias. The test treatment (OVX OZ) was performed with an intraperitoneal application of ozone at a concentration of 0.7mg/kg, in the following periods: immediate PO, 2nd, 4th, 6th, 8th, 10th, 12th postoperative day. The control (OVX SAL) was performed with an intraperitoneal application of 1 ml of saline solution, in the same periods. Euthanasia times were at 14 and 42 days for histometric and immunohistochemical examinations. When analyzing the linear extent of bone-implant contact (ELCOI) and area of newly formed bone (AON), there was statistical significance only at 14 days (p<0.05) with higher values for OVX OZ. In the intragroup comparison OVX OZ, between 14 and 42 days, there was statistical significance. In the intragroup comparison SAL, between 14 and 42 days, there was a statistically significant difference, both for ELCOI and for AON. The antibody against osteocalcin, in the period of 14 days, presented score 2 (moderate marking) for the OVX OZ group, and score 1 (mild) for the OVX SAL group. At 42 days, it showed marking for the OVX OZ and OVX SAL groups. Ozone therapy positively modifies the biological repair mechanism in the first periods, through the measurement of growth factors in the cytoplasm. Some are osteoinductive, stimulating osteoprogenitor cells, differentiating them as an aid in osteogenesis. Thus, it was concluded that ozone therapy favored peri-implant bone repair in the early years.
Due to its properties, ozone therapy appears as an interesting method for the analysis of peri-implant bone repair, especially in low-density bones, where the aim is to reduce the rate of bone-integrating implants. objective of the present study analyzed the effect of therapy on peri-implant bone repair in peri-implant rats the effect of inducing osteoporosis. For this, 10 rats, adults, with 6 months of age were used. After the bilateral ovariectomy procedure and a 90-day wait for the induction of osteoporosis, a surgery was performed to install the implants, and each animal had 1 implant in one of its installed tibias. The test treatment (OVX OZ) was performed with an intraperitoneal application of ozone at a concentration of 0.7mg/kg, in the following periods: immediate PO, 2nd, 4th, 6th, 8th, 10th, 12th postoperative day. The control (OVX SAL) was performed with an intraperitoneal application of 1 ml of saline solution, in the same periods. Euthanasia times were at 14 and 42 days for histometric and immunohistochemical examinations. When analyzing the linear extent of bone-implant contact (ELCOI) and area of newly formed bone (AON), there was statistical significance only at 14 days (p<0.05) with higher values for OVX OZ. In the intragroup comparison OVX OZ, between 14 and 42 days, there was statistical significance. In the intragroup comparison SAL, between 14 and 42 days, there was a statistically significant difference, both for ELCOI and for AON. The antibody against osteocalcin, in the period of 14 days, presented score 2 (moderate marking) for the OVX OZ group, and score 1 (mild) for the OVX SAL group. At 42 days, it showed marking for the OVX OZ and OVX SAL groups. Ozone therapy positively modifies the biological repair mechanism in the first periods, through the measurement of growth factors in the cytoplasm. Some are osteoinductive, stimulating osteoprogenitor cells, differentiating them as an aid in osteogenesis. Thus, it was concluded that ozone therapy favored peri-implant bone repair in the early years.
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Palavras-chave
Ozônio, Osteoporose, Implantação dentária endo-óssea, Ozone