Análise comparativa do processo de reparo ósseo alveolar em camundongos machos das linhagens 129Sv/Ev, C57Bl/6J e BALB/c
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Data
2022-11-11
Autores
Roseno, Ana Carolyna Becher [UNESP]
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Universidade Estadual Paulista (Unesp)
Resumo
O uso de pequenos roedores em estudos para investigação de eventos aplicados à
saúde humana já se encontra estabelecido, principalmente com o auxílio dos
camundongos. A variedade de linhagens disponíveis nos permite selecionar o animal
adequado para determinado alvo de estudo. Deste modo, o presente estudo teve
como objetivo caracterizar o processo de reparo ósseo alveolar de três linhagens de
camundongos, 129Sv/Ev, C57BL/6J e BALB/c, devido às diferenças na capacidade
de resposta inflamatória. Para tanto, foram utilizados 60 camundongos (n=20 para
cada linhagem) machos, com idade de 12 semanas, os quais foram submetidos à
exodontia do incisivo superior direito. Após 7 e 30 dias, os animais foram submetidos
à eutanásia e suas maxilas com os alvéolos em reparação removidas para análises
histopatológica descritiva e histomorfométrica, imuno-histoquímica e análise de
birrefringência. Apesar da reparação satisfatória de todos os alvéolos, a
histomorfometria revelou um aumento significativo de matriz óssea nos animais
129Sv/Ev após 30 dias, comparando-se com os C57Bl/6J e BALB/c. A
imunomarcação para TGF-β revelou-se aumentada nos animais C57Bl/6J e BALB/c
aos 7 dias em relação ao 129Sv/Ev. De modo geral, o grupo C57Bl/6J revelou
aumento significativo do número de células iNOS+. No entanto, considerando-se os
leucócitos positivos, os BALB/c apresentaram maior número destas células ao final do
reparo. Com relação ao TRAP, observou-se maior número de células positivas nos
animais 129Sv/Ev em relação aos BALB/c aos 7 dias. Não foram detectadas
diferenças significativas entre os grupos com relação aos anticorpos RANKL, RUNX 2 e COX-2. A partir dos resultados obtidos, pode-se concluir que há diferenças no
perfil inflamatório durante o curso de reparo intramembranoso alveolar entre as
linhagens de camundongos estudadas que interferem tanto na resposta inflamatória
esperada do modelo experimental, assim como na quantidade, porém, não na
qualidade da matriz óssea formada no final do reparo.
The use of small rodents for the investigation of the events correlated with human health is already established, especially when it comes on mice. The variety of available strain enables us to choose the adequate animal model for a specific research theme. Thus, the present study aimed to characterize the dental socket healing process of three different mice breeds, 129Sv/Ev, C57Bl/6J, and BALB/c, considering their differences in inflammatory response. Sixty male mice were used (n=20 for each breed), 12 weeks old, and underwent tooth extraction procedure for the removal of the upper right incisor. After 7, and 30 days post-operatory, they were euthanized and their maxillae with the healing sockets were removed for histology, histomorphometry, immunohistochemistry, and birefringence analysis. Besides the satisfactory repair of all dental sockets, histomorphometry revealed a significant increase of bone matrix in the 129Sv/Ev after 30 days, when compared with C57Bl/6J and BALB/c. Immunolabeling for TGF-B was increased in the C57Bl/6J and BALB/c mice at day 7, in comparison to the 129Sv/Ev. In general, C57Bl/6Jrevealed increased number of iNOS+ cells. However, considering the iNOS+ leukocytes, BALB/c presented the higher number of these cells at the end of the healing process. As for TRAP, higher number of positive cells was observed in 129Sv/Ev when compared with BALB/c at day 7. No significant differences were detected among the groups considering RANKL, RUNX-2, and COX-2. Birefringence analysis did not revealed significant difference among the groups, as well. From these results, it was concluded that there are differences considering the inflammatory profile among the breeds, during the intramembranous socket healing, which interfere in the inflammatory response of the experimental model, as well as in its intensity, but not in the quality of the bone matrix at the end of the healing process.
The use of small rodents for the investigation of the events correlated with human health is already established, especially when it comes on mice. The variety of available strain enables us to choose the adequate animal model for a specific research theme. Thus, the present study aimed to characterize the dental socket healing process of three different mice breeds, 129Sv/Ev, C57Bl/6J, and BALB/c, considering their differences in inflammatory response. Sixty male mice were used (n=20 for each breed), 12 weeks old, and underwent tooth extraction procedure for the removal of the upper right incisor. After 7, and 30 days post-operatory, they were euthanized and their maxillae with the healing sockets were removed for histology, histomorphometry, immunohistochemistry, and birefringence analysis. Besides the satisfactory repair of all dental sockets, histomorphometry revealed a significant increase of bone matrix in the 129Sv/Ev after 30 days, when compared with C57Bl/6J and BALB/c. Immunolabeling for TGF-B was increased in the C57Bl/6J and BALB/c mice at day 7, in comparison to the 129Sv/Ev. In general, C57Bl/6Jrevealed increased number of iNOS+ cells. However, considering the iNOS+ leukocytes, BALB/c presented the higher number of these cells at the end of the healing process. As for TRAP, higher number of positive cells was observed in 129Sv/Ev when compared with BALB/c at day 7. No significant differences were detected among the groups considering RANKL, RUNX-2, and COX-2. Birefringence analysis did not revealed significant difference among the groups, as well. From these results, it was concluded that there are differences considering the inflammatory profile among the breeds, during the intramembranous socket healing, which interfere in the inflammatory response of the experimental model, as well as in its intensity, but not in the quality of the bone matrix at the end of the healing process.
Descrição
Palavras-chave
Camundongos, Inflamação, Modelos animais, Mice