Avaliação da associação do gel de colágeno e biovidro f18® na cicatrização de feridas cutâneas não contaminadas em ratos Wistar
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Data
2022-12-20
Autores
Silva, Bruna Martins da
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
O biovidro F18® é um biomaterial cerâmico, o qual possui propriedades anti-biofime, angiogênica, vasodilatadora e osteocondutora. Objetiva-se com o presente estudo realizar avaliações clínica, termográfica, tensiométrica, morfométrica, histológica e imunoistoquímica de feridas cutâneas de ratos Wistar convencional tratadas com biovidro F18®. Foram utilizados 112 ratos Wistar convencional, machos adultos e hígidos. Após a anestesia geral, em cada animal foi efetuada uma ferida de 3 cm de diâmetro ne região dorsal. Os ratos foram divididos aleatoriamente em quatro grupos de 28 animais, de acordo com o tratamento: G1 - solução salina (0,9%); G2 - pomada cicatrizante; G3 - gel de colágeno; G4 – mistura do pó de biovidro F18® e gel de colágeno. A avaliação clínica incluiu a mensuração da massa corpórea, temperatura corpórea e a avaliação macroscópica da ferida. A avaliação da temperatura superficial das feridas cutâneas foi realizada pela tomografia infravermelha, e a tensiometria foi avaliada pela força de tensão e deformidade máxima. Para realização da avaliação histológica e imunoistoquímica, eutanásias foram realizadas após indução anestésica, e biópsias excisionais foram realizadas em torno da lesão. O tecido de granulação apresentou variações significativas ao longo dos momentos e entre os grupos. A temperatura superficial das feridas tratadas com a solução salina (0,9%) apresentou aumento significativo ao longo dos momentos e entre os grupos. Os valores das áreas e contração das feridas dos demonstraram redução e aumento significativo, respectivamente, ao longo dos momentos. Foi identificado aumento significativo no número de células totais e aumento significativo para colágeno tipo I na ferida tratado com biovidro F18®. Concluiu-se que o biovidro F18® demonstrou efeitos benéficos na cicatrização de feridas cutâneas, destacando sua ação na indução de maior perfusão sanguínea, aumento da força de tensão, diminuição da área da ferida e aumento da taxa de oclusão, e diminuição da quantidade de células de reação inflamatória e estimulo na produção de colágeno tipo I.
he F18® bioglass is a ceramic biomaterial, which has anti-biofilm, angiogenic, vasodilator and osteoconductive properties. The aim of this study is to carry out clinical, thermographic, tensiometric, morphometric, histological and immunohistochemical evaluations of skin wounds in conventional Wistar rats treated with F18® bioglass. 112 conventional Wistar rats, adult and healthy males, were used. After general anesthesia, a wound measuring 3 cm in diameter was made on the dorsal region in each animal. The rats were randomly divided into four groups of 28 animals, according to the treatment: G1 - saline solution (0.9%); G2 - healing ointment; G3 - collagen gel; G4 – mixture of F18® bioglass powder and collagen gel. Clinical evaluation included measurement of body mass, body temperature and macroscopic evaluation of the wound. The evaluation of the superficial temperature of the cutaneous wounds was performed by infrared tomography, and the tensiometry was evaluated by the tension force and maximum deformity. To carry out the histological and immunohistochemical evaluation, euthanasia was performed after anesthetic induction, and excisional biopsies were performed around the lesion. Granulation tissue showed significant variations over time and between groups. The surface temperature of the wounds treated with saline solution (0.9%) showed a significant increase over time and between groups. The values of the areas and contraction of the wounds showed a significant reduction and increase, respectively, over time. A significant increase in the number of total cells and a significant increase in type I collagen was identified in the wound treated with F18® bioglass. It was concluded that the F18® bioglass demonstrated beneficial effects in the healing of cutaneous wounds, highlighting its action in inducing greater blood perfusion, increasing the tension force, decreasing the wound area and increasing the occlusion rate, and decreasing the amount of inflammatory reaction cells and stimulation of type I collagen production.
he F18® bioglass is a ceramic biomaterial, which has anti-biofilm, angiogenic, vasodilator and osteoconductive properties. The aim of this study is to carry out clinical, thermographic, tensiometric, morphometric, histological and immunohistochemical evaluations of skin wounds in conventional Wistar rats treated with F18® bioglass. 112 conventional Wistar rats, adult and healthy males, were used. After general anesthesia, a wound measuring 3 cm in diameter was made on the dorsal region in each animal. The rats were randomly divided into four groups of 28 animals, according to the treatment: G1 - saline solution (0.9%); G2 - healing ointment; G3 - collagen gel; G4 – mixture of F18® bioglass powder and collagen gel. Clinical evaluation included measurement of body mass, body temperature and macroscopic evaluation of the wound. The evaluation of the superficial temperature of the cutaneous wounds was performed by infrared tomography, and the tensiometry was evaluated by the tension force and maximum deformity. To carry out the histological and immunohistochemical evaluation, euthanasia was performed after anesthetic induction, and excisional biopsies were performed around the lesion. Granulation tissue showed significant variations over time and between groups. The surface temperature of the wounds treated with saline solution (0.9%) showed a significant increase over time and between groups. The values of the areas and contraction of the wounds showed a significant reduction and increase, respectively, over time. A significant increase in the number of total cells and a significant increase in type I collagen was identified in the wound treated with F18® bioglass. It was concluded that the F18® bioglass demonstrated beneficial effects in the healing of cutaneous wounds, highlighting its action in inducing greater blood perfusion, increasing the tension force, decreasing the wound area and increasing the occlusion rate, and decreasing the amount of inflammatory reaction cells and stimulation of type I collagen production.
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Palavras-chave
Tecido cicatricial, Pele, Trauma, Angiogênese, Cicatrização, Cicatriz, Ferimentos e lesões, Biomateriais, Biovidro