Growth Curve, Morphological and Molecular Characterization of Two Strains of Trypanosoma cruzi (Kinetoplastida, Trypanosomatidae) isolated from Triatoma sherlocki (Hemiptera, Reduviidae, Triatominae)
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Data
2022-01-01
Autores
Kunii, Gabriela Kinue Watase [UNESP]
Falcone, Rossana [UNESP]
Clementino, Leandro da Costa [UNESP]
Rosa, João Aristeu da[UNESP]
Nascimento, Juliana Damieli
Belintani, Tiago
Oliveira, Jader de
Ribeiro, Aline Rimoldi
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Resumo
Background: Trypanosoma cruzi presents great variability in morphology, virulence, pathogenicity, avoidance of the host immune system, and antigenic constitution, associated with different clinical manifestations of the disease. Methods: Two strains of T. cruzi were cultivated in liver infusion tryptose to determine growth kinetics, morphometry and molecular characterization using restriction fragment length polymorphism polymerase chain reaction. Results: The biological parameters showed sharp growth by the 7th day. Morphologically, both strains showed short and thin forms and were classified as Group I. Conclusion: Group TcI presents cardiac manifestations and T. sherlocki is adapting to the home environment, requiring attention to future problems.
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Chagas disease, Epidemiology, Trypanosoma cruzi, Vector biology
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Revista da Sociedade Brasileira de Medicina Tropical, v. 55.