Kinetic aspects of rat (Rattus-norvegicus albinus) submandibular glands lactic-dehydrogenase
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Data
1994-01-01
Autores
Sassaki, K. T.
Rosa, C. D.
Cesar, M. D.
Rosa, R.
Título da Revista
ISSN da Revista
Título de Volume
Editor
Inst Tecnologia Parana
Resumo
Submandibular glands of male rats were homogenized with 33 mM sodium potassium phosphate buffer, pH 6.5, containing 1 mM MgCl2 and 0.1 mM DTT and purified with ammonium sulphate, phosphocellulose chromatography, eluted with KC1 0.5 M, followed by Blue Sepharose CL-6B chromatography, eluted with NADH 0.5 mM. The enzyme kepts stable for 60 days when stored at -15-degrees-C in 33 mM phosphate buffer. In other experiment the enzyme was purified by oxamate-agarose chromatography from a crude extract of submandibular gland and the results obtained were better than by phosphocellulose and Sepharose CL-6B chromatography. The Km values for pyruvate. NADH, lactate and NAD+ were established. Sodium oxamate at 0.1 and 0.9 mM concentrations inhibited the LDH activity by 40 and 85%, respectively (competitive); with sodium oxalate the inhibition was of 30% (uncompetitive) and with 3-acetyl pyridine adenine dinucleotide was 80%.
Descrição
Palavras-chave
LACTIC DEHYDROGENASE, SUBMANDIBULAR GLAND, SALIVARY GLAND
Como citar
Arquivos de Biologia E Tecnologia. Curitiba-Paraná: Inst Tecnologia Parana, v. 37, n. 2, p. 333-343, 1994.