Macrophages and mast cells control the neutrophil migration induced by dentin proteins
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Data
2005-01-01
Autores
Silva, T. A.
Lara, V. S.
Silva, J. S.
Oliveira, SHP
Butler, W. T.
Cunha, F. Q.
Título da Revista
ISSN da Revista
Título de Volume
Editor
Int Amer Assoc Dental Researchi
Resumo
Dentin sialoprotein (DSP) and dentin phosphoprotein (DPP), the major dentin proteins, have been shown to induce neutrophil migration through release of IL-1beta, TNF-alpha, MIP-2, and KC. However, the sources of these mediators were not determined. Here, the roles of macrophages and mast cells (MC) in dentin-induced neutrophil accumulation were investigated. Peritoneal MC depletion or the enhancement of macrophage population increased DSP- and DPP-induced neutrophil extravasation. Moreover, supernatants from DSP- and DPP-stimulated macrophages caused neutrophil migration. The release of neutrophil chemotactic factor by macrophages was inhibited by dexamethasone or the supernatant of DSP- treated MC. Consistently, dexamethasone and the MC supernatant inhibited the production of IL-1beta, TNF-alpha, and MIP-2 by macrophages. This inhibitory activity of the DSP- stimulated MC was neutralized by anti-IL-4 and anti-IL-10 antibodies. These results indicate that dentin induces the release of the neutrophil chemotactic substance(s) by macrophages, which are down-modulated by MC-derived IL-4 and IL-10.
Descrição
Palavras-chave
neutrophil chemotaxis, DSP, DPP
Como citar
Journal of Dental Research. Alexandria: Int Amer Associação Dental Researchi A D R/a A D R, v. 84, n. 1, p. 79-83, 2005.