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dc.contributor.authorVoltan, A. R. [UNESP]
dc.contributor.authorDonofrio, F. [UNESP]
dc.contributor.authorMiranda, E. T. [UNESP]
dc.contributor.authorMoraes, R. A. [UNESP]
dc.contributor.authorMendes-Giannini, Maria José Soares [UNESP]
dc.date.accessioned2014-05-27T11:23:41Z
dc.date.available2014-05-27T11:23:41Z
dc.date.issued2008-10-28
dc.identifierhttp://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/view/439
dc.identifier.citationRevista de Ciencias Farmaceuticas Basica e Aplicada, v. 29, n. 1, p. 97-106, 2008.
dc.identifier.issn1808-4532
dc.identifier.issn2179-443X
dc.identifier.urihttp://hdl.handle.net/11449/70618
dc.description.abstractP. brasiliensis parasitizes various human tissues and proteinases exported by this fungus may allow it to metabolize and invade host tissues. The influence of the culture medium on the production of proteinases by P. brasiliensis isolates was studied and the export of these enzymes was followed as a function of culture time. The fungus was grown in neopeptone, BSA, elastin or collagen medium. The culture medium was assayed for azocollytic, elastinolytic and caseinolytic activity. Proteolytic activity was also analysed by electrophoresis of the culture medium on gelatin and casein substrate gels. P. brasiliensis expressed relatively high levels of azocoll, elastin and casein degrading activity in all types of medium, except in neopeptone medium. Generally, expression of azocollytic activity peaked during the third week of culture and caseinolytic activity during the fourth week of culture. Azocollytic activity was highest at pH 4.0 and caseinolytic activity at pH 8.0. Elastinolytic activity was also highest at pH 8.0. This activity, as well as the others, may provide the fungus with a source of carbon and nitrogen and may also be responsible for the invasion of host tissues, such as pulmonary elastic fiber, by P. brasiliensis.en
dc.format.extent97-106
dc.language.isoeng
dc.relation.ispartofRevista de Ciências Farmacêuticas Básica e Aplicada
dc.sourceScopus
dc.subjectCollagenolytic activity
dc.subjectElastinolytic activity
dc.subjectParacoccidioides brasiliensis
dc.subjectParacoccidioidomycosis
dc.subjectProteolytic activity
dc.subjectcarbon
dc.subjectcasein
dc.subjectelastin
dc.subjectgelatin
dc.subjectnitrogen
dc.subjectculture medium
dc.subjectelastic fiber
dc.subjectelastolysis
dc.subjectfungus culture
dc.subjectfungus growth
dc.subjectfungus isolation
dc.subjectnonhuman
dc.subjectpH
dc.subjectpolyacrylamide gel electrophoresis
dc.subjectprotein degradation
dc.subjectprotein induction
dc.subjectprotein secretion
dc.subjectprotein synthesis
dc.titleInduction and secretion of elastinolytic and proteolytic activity in cultures of Paracoccidioides brasiliensisen
dc.typeArtigo
dcterms.licensehttp://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/about
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.description.affiliationLaboratório de Micologia Clínica Departamento de Análises Clínicas Universidade Estadual Paulista, UNESP, Araraquara, SP
dc.description.affiliationLaboratório de Micologia Clínica Departamento de Análises Clínicas Universidade Estadual Paulista, UNESP, Rua Expedicionários do Brasil, 1621, CEP: 14801-902 - Araraquara - SP
dc.description.affiliationUnespLaboratório de Micologia Clínica Departamento de Análises Clínicas Universidade Estadual Paulista, UNESP, Araraquara, SP
dc.description.affiliationUnespLaboratório de Micologia Clínica Departamento de Análises Clínicas Universidade Estadual Paulista, UNESP, Rua Expedicionários do Brasil, 1621, CEP: 14801-902 - Araraquara - SP
dc.rights.accessRightsAcesso aberto
dc.identifier.scopus2-s2.0-54349119242
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Ciências Farmacêuticas, Araraquarapt
dc.identifier.file2-s2.0-54349119242.pdf
dc.identifier.orcid0000-0002-8059-0826
unesp.author.orcid0000-0002-8059-0826[5]
dc.relation.ispartofsjr0,131
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