Estudo histomorfométrico do processo de reparo de incisivos de ratos diabéticos submetidos à luxação extrusiva
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Data
2012-02-10
Autores
Nogueira, Lamis Meorin [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
Traumas na região oral ocorrem com freqüência e representam 5% de todos os ferimentos em que indivíduos procuram tratamento. A prevalência dos traumas de luxação em crianças e pacientes jovens, desperta uma preocupação com a condição do tecido pulpar desses dentes que geralmente apresentam rizogênese incompleta. Outro fator importante é o aumento da prevalência de indivíduos com alterações sistêmicas, principalmente o Diabetes Melittus, que pode levar a um atraso na reparação tecidual e predispor o indivíduo a infecções. Portanto o objetivo deste trabalho foi estudar a reparação periodontal e pulpar de ratos diabéticos e normais submetidos à luxação extrusiva do incisivo superior. Para tanto foram utilizados 30 animais divididos em 3 grupos de 10. No grupo I (controle) não foi realizado nenhum procedimento cirúrgico. Para a indução do Diabetes no grupo III, utilizou-se a estreptozotocina diluída em tampão citrato, aplicada na veia peniana. No grupo II utilizou-se apenas o tampão citrato. Constatada a condição de Diabetes no grupo III, realizou-se neste grupo e no grupo II a luxação extrusiva em 2mm do incisivo superior direito. Após 60 dias os animais foram submetidos à eutanásia e perfusão e as peças obtidas, processadas para análise pela Hematoxilina e Eosina. Foi realizada uma análise histomorfométrica dos resultados. Entre os parâmetros analisados, as maiores alterações ocorreram na inserção do epitélio gengival, tecido pulpar e ligamento periodontal, que se apresentaram mais comprometido nos animais diabéticos. De uma forma geral, não foram encontradas diferenças estatisticamente significantes entre os grupos II e III
Trauma to the oral region occurs frequently and comprises 5% of all injuries for which people seek treatment. The prevalence of dislocation trauma in children and young patients raises a concern about the condition of the pulp tissue of teeth that generally have incomplete root formation. Another important factor is the increasing prevalence of individuals with systemic conditions, especially diabetes mellitus, which can lead to a delay in tissue repair and predispose individuals to infection. Therefore, the purpose was to study the repair of periodontal and pulp tissue of normal and diabetic rats submitted to extrusive luxation of the maxillary incisor. Therefore, we use 30 animals divided into 3 groups of 10. In group I (control) was not carried out any surgical procedure. For the induction of Diabetes in group III was used the streptozotocin diluted in citrate buffer applied to the penile vein. Group II was used only citrate buffer. Given the condition of diabetes in group III, in this group and group II was performed the extrusive luxation of 2mm in the right maxillary incisor. After 60 days the animals were euthanized and perfusion and the specimens were processed for analysis by hematoxylin and eosin. We performed a histomorphometric analysis of the results. Among the parameters analyzed, the largest changes occurred in the insertion of the gingival epithelium, dental pulp and periodontal ligament, which were more impaired in diabetic animals. Overall, there were no statistically significant differences between groups II and III
Trauma to the oral region occurs frequently and comprises 5% of all injuries for which people seek treatment. The prevalence of dislocation trauma in children and young patients raises a concern about the condition of the pulp tissue of teeth that generally have incomplete root formation. Another important factor is the increasing prevalence of individuals with systemic conditions, especially diabetes mellitus, which can lead to a delay in tissue repair and predispose individuals to infection. Therefore, the purpose was to study the repair of periodontal and pulp tissue of normal and diabetic rats submitted to extrusive luxation of the maxillary incisor. Therefore, we use 30 animals divided into 3 groups of 10. In group I (control) was not carried out any surgical procedure. For the induction of Diabetes in group III was used the streptozotocin diluted in citrate buffer applied to the penile vein. Group II was used only citrate buffer. Given the condition of diabetes in group III, in this group and group II was performed the extrusive luxation of 2mm in the right maxillary incisor. After 60 days the animals were euthanized and perfusion and the specimens were processed for analysis by hematoxylin and eosin. We performed a histomorphometric analysis of the results. Among the parameters analyzed, the largest changes occurred in the insertion of the gingival epithelium, dental pulp and periodontal ligament, which were more impaired in diabetic animals. Overall, there were no statistically significant differences between groups II and III
Descrição
Palavras-chave
Diabetes, Reabsorção da raiz (Dentes), Ligamento periodontal
Como citar
NOGUEIRA, Lamis Meorin. Estudo histomorfométrico do processo de reparo de incisivos de ratos diabéticos submetidos à luxação extrusiva. 2012. 68 f. Dissertação (mestrado) - Universidade Estadual Paulista, Faculdade de Odontologia de Araçatuba, 2012.