Use of giant unilamellar lipid vesicles as antioxidant carriers in in vitro culture medium of bovine embryos

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dc.contributor.authorRossi, Luana Teixeira Rodrigues [UNESP]
dc.contributor.authorNunes, Giovana Barros [UNESP]
dc.contributor.authorda Silva, Cíntia Rodrigues [UNESP]
dc.contributor.authorde Rossi, Hugo [UNESP]
dc.contributor.authordos Santos, Priscila Helena [UNESP]
dc.contributor.authorNogueira, Marcelo Fábio Gouveia [UNESP]
dc.contributor.authorAoki, Pedro Henrique Benites [UNESP]
dc.contributor.authorMingoti, Gisele Zoccal [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2023-03-01T20:54:06Z
dc.date.available2023-03-01T20:54:06Z
dc.date.issued2022-12-01
dc.description.abstractGiant unilamellar vesicles (GUVs) are composed of lipophilic layers and are sensitive to the action of reactive oxygen species (ROS). The use of GUVs as microcarriers of biological macromolecules is particularly interesting since ROS produced by gametes or embryos during in vitro culture can induce the opening of pores in the membrane of these vesicles and cause the release of their content. This study investigated the behavior of GUVs [composed of 2-dioleoyl-sn-glycero-3-phosphocholine and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl)] in co-culture with in vitro produced bovine embryos, as well as their embryotoxicity and effectiveness as cysteine carriers in culture medium. Embryonic developmental rates were unaffected, demonstrating the absence of toxicity of GUVs co-cultured with the embryos. No increase of intracellular ROS levels was observed in the embryos co-cultured with GUVs, indicating that the higher lipid content of the culture environment resulting from the lipid composition of the GUV membrane itself did not increase oxidative stress. Variations in the diameter and number of GUVs demonstrated their sensitivity to ROS produced by embryos cultured under conditions that generate oxidative stress. Encapsulation of cysteine in GUVs was found to be more effective in controlling the production of ROS in embryonic cells than direct dilution of this antioxidant in the medium. In conclusion, the use of GUVs in in vitro culture was found to be safe since these vesicles did not promote toxic effects nor did they increase intracellular ROS concentrations in the embryos. GUVs were sensitive to oxidative stress, which resulted in structural changes in response to the action of ROS. The possible slow release of cysteine into the culture medium by GUV rupture would therefore favor the gradual supply of cysteine, prolonging its presence in the medium. Thus, the main implication of the use of GUVs as cysteine microcarriers is the greater effectiveness in preventing the intracytoplasmic increase of ROS in in vitro produced bovine embryos.en
dc.description.affiliationLaboratory of Reproductive Physiology Department of Animal Production and Health School of Veterinary Medicine São Paulo State University (UNESP), Clóvis Pestana Street, 793, Araçatuba Campus
dc.description.affiliationGraduate Program in Veterinary Medicine Department of Animal Reproduction School of Agrarian and Veterinary Sciences São Paulo State University (UNESP), Campus Jaboticabal
dc.description.affiliationDepartment of Biological Sciences School of Sciences and Languages São Paulo State University (UNESP), Campus Assis
dc.description.affiliationGraduate Program in Pharmacology and Biotechnology Institute of Biosciences São Paulo State University (UNESP), Campus Botucatu
dc.description.affiliationDepartment of Biotechnology School of Sciences and Languages São Paulo State University (UNESP), Campus Assis
dc.description.affiliationUnespLaboratory of Reproductive Physiology Department of Animal Production and Health School of Veterinary Medicine São Paulo State University (UNESP), Clóvis Pestana Street, 793, Araçatuba Campus
dc.description.affiliationUnespGraduate Program in Veterinary Medicine Department of Animal Reproduction School of Agrarian and Veterinary Sciences São Paulo State University (UNESP), Campus Jaboticabal
dc.description.affiliationUnespDepartment of Biological Sciences School of Sciences and Languages São Paulo State University (UNESP), Campus Assis
dc.description.affiliationUnespGraduate Program in Pharmacology and Biotechnology Institute of Biosciences São Paulo State University (UNESP), Campus Botucatu
dc.description.affiliationUnespDepartment of Biotechnology School of Sciences and Languages São Paulo State University (UNESP), Campus Assis
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIdFAPESP: 2012/50533-2
dc.description.sponsorshipIdFAPESP: 2018/16713-0
dc.description.sponsorshipIdFAPESP: 2019/11174-6
dc.description.sponsorshipIdCNPq: 314136/2018-5
dc.description.sponsorshipIdCNPq: 403713/2016-1
dc.description.sponsorshipIdCAPES: Finance Code 001
dc.identifierhttp://dx.doi.org/10.1038/s41598-022-14688-8
dc.identifier.citationScientific Reports, v. 12, n. 1, 2022.
dc.identifier.doi10.1038/s41598-022-14688-8
dc.identifier.issn2045-2322
dc.identifier.scopus2-s2.0-85133257406
dc.identifier.urihttp://hdl.handle.net/11449/241263
dc.language.isoeng
dc.relation.ispartofScientific Reports
dc.sourceScopus
dc.titleUse of giant unilamellar lipid vesicles as antioxidant carriers in in vitro culture medium of bovine embryosen
dc.typeArtigo
unesp.author.orcid0000-0002-3818-7347[3]
unesp.author.orcid0000-0002-2239-9652[6]
unesp.author.orcid0000-0003-4701-6408[7]
unesp.author.orcid0000-0002-3059-4458[8]
unesp.departmentMedicina Veterinária Preventiva e Reprodução Animal - FCAVpt
unesp.departmentCiências Biológicas - FCLASpt

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Artigos - Ciências Básicas - FOA
Artigos - Farmacologia - IBB
Assis - FCLAS - Faculdade de Ciências e Letras
Jaboticabal - FCAV - Faculdade de Ciências Agrárias e Veterinárias