Low Concentration of 5-Fluorouracil Increases the Effectiveness of Tumor RNA to Activate Murine Dendritic Cells

dc.contributor.authorCamargo, Marcela Rodrigues de [UNESP]
dc.contributor.authorGorgulho, Carolina Mendonca [UNESP]
dc.contributor.authorRodrigues, Cecilia Pessoa [UNESP]
dc.contributor.authorPenitenti, Marcimara
dc.contributor.authorLongo Frederico, Juliana Cristina [UNESP]
dc.contributor.authorMarchesan Rodrigues, Maria Aparecida [UNESP]
dc.contributor.authorKaneno, Ramon [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionHosp Amaral Carvalho
dc.date.accessioned2018-11-26T17:41:53Z
dc.date.available2018-11-26T17:41:53Z
dc.date.issued2017-10-01
dc.description.abstractAim: Considering the central role of dendritic cells (DCs) on the development of an antitumor immune response, in this study we used a murine model to evaluate how DC transfection with drug-treated tumor cell RNA changes their phenotype, and whether transfection enhances the in vivo effectiveness of a DC-based antitumor vaccine. Materials and Methods: MC-38 colorectal tumor cells were pretreated with the minimum effective concentration of 5-fluorouracil (5-FU), then their total RNA was extracted and transfected into DCs. These DCs were inoculated into C57Bl/6 mice bearing subcutaneous MC-38 tumor. Results: DC transfection with drug-treated tumor RNA increases the percentages of CD40(+) (from 37.6% to 61.4%), CD86(+) (from 39.8% to 53.4%), and major histocompatibility complex class II+ (from 51.2% to 75.3%) cells, whereas significantly increases the in vivo generation of interferon-c producer lymphocytes. Conclusion: These results reinforce our view that treatment of tumor cells with 5-FU induces transcriptional changes that can be transferred to DCs by RNA transfection, enhancing their ability to stimulate an antitumor response.en
dc.description.affiliationSao Paulo State Univ UNESP, Sch Med Botucatu, Dept Pathol, Botucatu, SP, Brazil
dc.description.affiliationSao Paulo State Univ UNESP, Inst Biosci Botucatu, Dept Microbiol & Immunol, Botucatu, SP, Brazil
dc.description.affiliationHosp Amaral Carvalho, Lab Flow Cytometry Jau, Jau, Brazil
dc.description.affiliationUnespSao Paulo State Univ UNESP, Sch Med Botucatu, Dept Pathol, Botucatu, SP, Brazil
dc.description.affiliationUnespSao Paulo State Univ UNESP, Inst Biosci Botucatu, Dept Microbiol & Immunol, Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipBrazilian Council of Research and Development
dc.description.sponsorshipIdFAPESP: 2009/18331-8
dc.description.sponsorshipIdFAPESP: 2010/06013-9
dc.description.sponsorshipIdFAPESP: 2011/19403-2
dc.description.sponsorshipIdFAPESP: 2011/05258-0
dc.description.sponsorshipIdBrazilian Council of Research and Development: CNPq: 13991/2013-2
dc.description.sponsorshipIdBrazilian Council of Research and Development: 303952/2010-5
dc.format.extent302-308
dc.identifierhttp://dx.doi.org/10.1089/cbr.2017.2259
dc.identifier.citationCancer Biotherapy And Radiopharmaceuticals. New Rochelle: Mary Ann Liebert, Inc, v. 32, n. 8, p. 302-308, 2017.
dc.identifier.doi10.1089/cbr.2017.2259
dc.identifier.issn1084-9785
dc.identifier.lattes8845835550637809
dc.identifier.orcid0000-0002-4292-3298
dc.identifier.urihttp://hdl.handle.net/11449/163407
dc.identifier.wosWOS:000413337900005
dc.language.isoeng
dc.publisherMary Ann Liebert, Inc
dc.relation.ispartofCancer Biotherapy And Radiopharmaceuticals
dc.relation.ispartofsjr0,567
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectchemotherapy
dc.subjectcolorectal cancer
dc.subjectdendritic cell
dc.subjectimmunomodulation
dc.subjectvaccine
dc.titleLow Concentration of 5-Fluorouracil Increases the Effectiveness of Tumor RNA to Activate Murine Dendritic Cellsen
dc.typeArtigo
dcterms.rightsHolderMary Ann Liebert, Inc
unesp.author.lattes8845835550637809[7]
unesp.author.orcid0000-0002-4292-3298[7]

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