Hepatoprotective and anti-inflammatory effects of silibinin on experimental preeclampsia induced by 1-NAME in rats

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dc.contributor.authorSouza, Camila Oliveira de [UNESP]
dc.contributor.authorPeraçoli, Maria Terezinha Serrão [UNESP]
dc.contributor.authorWeel, Ingrid Cristina [UNESP]
dc.contributor.authorBannwart-Castro, Camila F. [UNESP]
dc.contributor.authorRomao, Mariana [UNESP]
dc.contributor.authorNakaira-Takahagi, Erika [UNESP]
dc.contributor.authorLopes de Medeiros, Leonardo Teixeira [UNESP]
dc.contributor.authorSilva, Marcia da [UNESP]
dc.contributor.authorPeraçoli, José Carlos [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:35:14Z
dc.date.available2014-05-20T13:35:14Z
dc.date.issued2012-09-04
dc.description.abstractAims: Inhibition of nitric oxide synthase with N-omega-nitro-L-arginine methyl ester (L-NAME) has been employed as an experimental model of human preeclampsia. This study determined the protective effect of silibinin, a flavonoid with anti-inflammatory and hepatoprotective properties on the deleterious effects observed in experimentally induced preeclampsia in rats.Main methods: Pregnant Wistar rats were treated during gestation (days 10-20) with L-NAME (70-80 mg/kg/day) in drinking water or with L-NAME plus silibinin (100 mg/kg/day, orally) starting at day 0, day 7 or day 14 of pregnancy. Systolic blood pressure was recorded from gestation days 0 to 21. A control group of pregnant non-treated rats was analyzed similarly. on day 21 the rats were euthanized and the following parameters were evaluated: proteinuria, platelet count, liver histopathology and reproductive outcome. Tumor necrosis factor-alpha (TNF-alpha), interleukin-1 (IL-1 beta), IL-6, IL-10 and interferon-gamma (IFN-gamma) were determined in liver homogenate by enzyme immunoassay.Key findings: In comparison with the L-NAME group the silibinin treatment reduced the values of systolic blood pressure, proteinuria, INF-alpha, IL-1 beta and IFN-gamma in liver, normalized the platelet count and improved fetal outcomes. Histopathological lesions in liver of the L-NAME group showed intense mononuclear inflammatory infiltrate and thickening of muscle tunica of arterial vessel, mainly in the periportal area. Silibinin treatment induced attenuation of periportal inflammatory infiltrate, showing an association between inflammatory infiltrate and TNF-alpha, IL-1 beta and IFN-gamma levels in liver homogenate.Significance: Silibinin administration to L-NAME-treated rats displays anti-inflammatory and immunomodulatoty actions that may contribute to its hepatoprotective effects and improve reproductive outcomes in experimental preeclampsia. (C) 2012 Elsevier B.V. All rights reserved.en
dc.description.affiliationSão Paulo State Univ, UNESP, Inst Biosci, Dept Microbiol & Immunol, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationSão Paulo State Univ, UNESP, Botucatu Med Sch, Dept Gynecol & Obstet, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ, UNESP, Inst Biosci, Dept Microbiol & Immunol, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ, UNESP, Botucatu Med Sch, Dept Gynecol & Obstet, BR-18618970 Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 06/07095-3
dc.format.extent159-165
dc.identifierhttp://dx.doi.org/10.1016/j.lfs.2012.06.036
dc.identifier.citationLife Sciences. Oxford: Pergamon-Elsevier B.V. Ltd, v. 91, n. 5-6, p. 159-165, 2012.
dc.identifier.doi10.1016/j.lfs.2012.06.036
dc.identifier.issn0024-3205
dc.identifier.lattes5240998569868081
dc.identifier.lattes8499437381595614
dc.identifier.lattes6486557387397806
dc.identifier.urihttp://hdl.handle.net/11449/12109
dc.identifier.wosWOS:000308123300002
dc.language.isoeng
dc.publisherPergamon-Elsevier B.V. Ltd
dc.relation.ispartofLife Sciences
dc.relation.ispartofjcr3.234
dc.relation.ispartofsjr1,071
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectCytokinesen
dc.subjectL-NAMEen
dc.subjectPreeclampsiaen
dc.subjectPregnant ratsen
dc.subjectSilibininen
dc.titleHepatoprotective and anti-inflammatory effects of silibinin on experimental preeclampsia induced by 1-NAME in ratsen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderPergamon-Elsevier B.V. Ltd
unesp.advisor.lattes6486557387397806
unesp.author.lattes5240998569868081
unesp.author.lattes8499437381595614
unesp.author.orcid0000-0002-8990-0237[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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Artigos - Microbiologia e Imunologia - IBB
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