Evaluation of three enrichment broths and five plating media for Salmonella detection in poultry

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dc.contributor.authorMores Rall, Vera Lúcia [UNESP]
dc.contributor.authorRall, Ricardo [UNESP]
dc.contributor.authorAragon, Lina Casale [UNESP]
dc.contributor.authorDa Silva, Márcia Guimarães [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2022-04-28T20:06:54Z
dc.date.available2022-04-28T20:06:54Z
dc.date.issued2005-04-01
dc.description.abstractWe evaluated the effectiveness of Selenite Cystine (SC), Tetrathionate Brilliant Green (TBG) and Rappaport Vassiliadis (RV) broths for Salmonella isolation. We also tested three classic plating media, Salmonella-Shigella Agar (SS), Brilliant Green Agar (BGA), Xylose Lysine Desoxycholate Agar (XLD) and two chromogenic agars, Rambach (RA) and CHROMagar Salmonella (CAS). Among 100 poultry carcasses, 29 were positive for Salmonella using all plating media combined. RV broth (69%) and TT broth (58.6%) were more effective than SC broth (24.1%). The chromogenic media gave better results than the classic ones with less false-positive colonies. The most effective isolation medium was CHROMagar, where Salmonella was identified in 23 (79.3%) of the 29 positive samples, followed by Rambach (48%). Positivity for Salmonella using classic media was 13.8% for BGA, 27.6% for SS and 34.5% for XLD.en
dc.description.affiliationDepartamento de Microbiologia e Imunologia Universidade Estadual Paulista, Botucatu, SP
dc.description.affiliationDepartamento de Patologia Universidade Estadual Paulista, Botucatu, SP
dc.description.affiliationDepartamento de Microbiologia e Imunologia Instituto de Biociências Universidade Estadual Paulista, Caixa Postal 510, 18618-000, Botucatu, São Paulo
dc.description.affiliationUnespDepartamento de Microbiologia e Imunologia Universidade Estadual Paulista, Botucatu, SP
dc.description.affiliationUnespDepartamento de Patologia Universidade Estadual Paulista, Botucatu, SP
dc.description.affiliationUnespDepartamento de Microbiologia e Imunologia Instituto de Biociências Universidade Estadual Paulista, Caixa Postal 510, 18618-000, Botucatu, São Paulo
dc.format.extent147-150
dc.identifier.citationBrazilian Journal of Microbiology, v. 36, n. 2, p. 147-150, 2005.
dc.identifier.issn1517-8382
dc.identifier.issn1678-4405
dc.identifier.scopus2-s2.0-31144478349
dc.identifier.urihttp://hdl.handle.net/11449/224695
dc.language.isoeng
dc.relation.ispartofBrazilian Journal of Microbiology
dc.sourceScopus
dc.subjectPlating media
dc.subjectPoultry
dc.subjectSalmonella
dc.subjectSelective enrichment
dc.titleEvaluation of three enrichment broths and five plating media for Salmonella detection in poultryen
dc.typeArtigo

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Artigos - Microbiologia e Imunologia - IBB
Artigos - Patologia - FMB