Publicação:
DNA methylation patterns in bladder cancer and washing cell sediments: a perspective for tumor recurrence detection

dc.contributor.authorNegraes, Priscilla D. [UNESP]
dc.contributor.authorFavaro, Francine P. [UNESP]
dc.contributor.authorCamargo, João Lauro Viana de [UNESP]
dc.contributor.authorOliveira, Maria Luiza Cotrim Sartor de [UNESP]
dc.contributor.authorGoldberg, José [UNESP]
dc.contributor.authorRainho, Claudia A. [UNESP]
dc.contributor.authorSalvadori, Daisy Maria Favero [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:37:23Z
dc.date.available2014-05-20T13:37:23Z
dc.date.issued2008-08-14
dc.description.abstractBackground: Epigenetic alterations are a hallmark of human cancer. In this study, we aimed to investigate whether aberrant DNA methylation of cancer-associated genes is related to urinary bladder cancer recurrence.Methods: A set of 4 genes, including CDH1 (E-cadherin), SFN (stratifin), RARB (retinoic acid receptor, beta) and RASSF1A (Ras association (RalGDS/AF-6) domain family 1), had their methylation patterns evaluated by MSP (Methylation-Specific Polymerase Chain Reaction) analysis in 49 fresh urinary bladder carcinoma tissues (including 14 cases paired with adjacent normal bladder epithelium, 3 squamous cell carcinomas and 2 adenocarcinomas) and 24 cell sediment samples from bladder washings of patients classified as cancer-free by cytological analysis (control group). A third set of samples included 39 archived tumor fragments and 23 matched washouts from 20 urinary bladder cancer patients in post-surgical monitoring. After genomic DNA isolation and sodium bisulfite modification, methylation patterns were determined and correlated with standard clinic-histopathological parameters.Results: CDH1 and SFN genes were methylated at high frequencies in bladder cancer as well as in paired normal adjacent tissue and exfoliated cells from cancer-free patients. Although no statistically significant differences were found between RARB and RASSF1A methylation and the clinical and histopathological parameters in bladder cancer, a sensitivity of 95% and a specificity of 71% were observed for RARB methylation (Fisher's Exact test (p < 0.0001; OR = 48.89) and, 58% and 17% (p < 0.05; OR = 0.29) for RASSF1A gene, respectively, in relation to the control group.Conclusion: Indistinct DNA hypermethylation of CDH1 and SFN genes between tumoral and normal urinary bladder samples suggests that these epigenetic features are not suitable biomarkers for urinary bladder cancer. However, RARB and RASSF1A gene methylation appears to be an initial event in urinary bladder carcinogenesis and should be considered as defining a panel of differentially methylated genes in this neoplasia in order to maximize the diagnostic coverage of epigenetic markers, especially in studies aiming at early recurrence detection.en
dc.description.affiliationSão Paulo State Univ, UNESP, Biosci Inst, Dept Genet, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationSão Paulo State Univ, UNESP, Botucatu Med Sch, Dept Pathol, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationSão Paulo State Univ, UNESP, Botucatu Med Sch, Dept Urol, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ, UNESP, Biosci Inst, Dept Genet, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ, UNESP, Botucatu Med Sch, Dept Pathol, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ, UNESP, Botucatu Med Sch, Dept Urol, BR-18618000 Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdFAPESP: 03/11730-8
dc.description.sponsorshipIdFAPESP: 04/00108-7
dc.format.extent12
dc.identifierhttp://dx.doi.org/10.1186/1471-2407-8-238
dc.identifier.citationBmc Cancer. London: Biomed Central Ltd., v. 8, p. 12, 2008.
dc.identifier.doi10.1186/1471-2407-8-238
dc.identifier.fileWOS000259072700002.pdf
dc.identifier.issn1471-2407
dc.identifier.lattes5051118752980903
dc.identifier.urihttp://hdl.handle.net/11449/12929
dc.identifier.wosWOS:000259072700002
dc.language.isoeng
dc.publisherBiomed Central Ltd.
dc.relation.ispartofBMC Cancer
dc.relation.ispartofjcr3.288
dc.relation.ispartofsjr1,464
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.titleDNA methylation patterns in bladder cancer and washing cell sediments: a perspective for tumor recurrence detectionen
dc.typeArtigo
dcterms.licensehttp://www.biomedcentral.com/about/license
dcterms.rightsHolderBiomed Central Ltd.
dspace.entity.typePublication
unesp.author.lattes5051118752980903
unesp.author.orcid0000-0003-3833-4172[3]
unesp.author.orcid0000-0002-0285-1162[6]
unesp.author.orcid0000-0001-9323-3134[7]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt
unesp.departmentPatologia - FMBpt
unesp.departmentUrologia - FMBpt
unesp.departmentGenética - IBBpt

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