Phytochemical study and evaluation of cytotoxicity, mutagenicity, cell cycle kinetics and gene expression of Bauhinia holophylla (Bong.) Steud. in HepG2 cells in vitro

Ribeiro, Diego Luis; Cilião, Heloísa Lizotti; Specian, Ana Flávia Leal; Serpeloni, Juliana Mara [UNESP]; De Oliveira, Marcelo Tempesta; Varanda, Eliana Aparecida [UNESP]; Vilegas, Wagner [UNESP]; Saldanha, Luiz Leonardo [UNESP]; Martínez-López, Wilner; Dokkedal, Anne Lígia [UNESP]; Cólus, Ilce Mara Syllos

Phytochemical study and evaluation of cytotoxicity, mutagenicity, cell cycle kinetics and gene expression of Bauhinia holophylla (Bong.) Steud. in HepG2 cells in vitro

Arquivos

2-s2.0-85037717319.pdf (1.03 MB)

Data

2018-04-01

Autores

Ribeiro, Diego Luis

Cilião, Heloísa Lizotti

Specian, Ana Flávia Leal

Serpeloni, Juliana Mara

De Oliveira, Marcelo Tempesta

Varanda, Eliana Aparecida

Vilegas, Wagner

Saldanha, Luiz Leonardo

Martínez-López, Wilner

Dokkedal, Anne Lígia

Tipo

Artigo

Direito de acesso

Acesso aberto

Resumo

Bauhinia holophylla (Bong.) Steud. (Fabaceae) is a plant used in Brazilian folk medicine to treat diabetes and inflammation. This study evaluated the phytochemical properties, cytotoxic, apoptotic, mutagenic/antimutagenic effects and alterations in gene expression (RNAm) in HepG2 cells treated with the B. holophylla extract. The phytochemical profile highlight the presence of flavonoids isorhamentin and quercetin derivates. The MTT assay was used to evaluate the cytotoxicity of different concentrations for different treatment times. Three concentrations (7.5, 15, 30 µg/mL) were chosen for assessment of apoptosis (AO/EB), mutagenicity (micronucleus), and cell cycle kinetics (flow cytometry). Thereafter, the concentration of 7.5 µg/mL was chosen to evaluate the protective effects against DNA damage induced by benzo[a]pyrene (B[a]P). At concentrations higher than 7.5 µg/mL (between 10 and 50 µg/mL), the extract was cytotoxic, induced apoptosis, and caused antiproliferative effects. However, it did not induce micronucleus and a reduction of apoptotic and micronucleated cells was observed in treatments that included the extract and B[a]P. The protective effect is attributable to the presence of flavonoids, described as antioxidants, inhibitors of DNA adduct and activators of detoxifying enzymes. The results of the present study such as absence of cytotoxic and mutagenic effects and protective effects against known carcinogens suggest that B. holophylla has potential for use soon as herbal medicine.