Characterization of myeloid or lymphoid acute leukemia by a chemiluminescence assay: Comparison with immunocytochemistry using an antimyeloperoxidase antibody
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Karger Publishers
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Resumo
A simple and sensitive chemiluminescence assay for the demonstration of the activity of intracellular myeloperoxidase (MPO) is described, which is useful for the distinction between myeloid and lymphoid commitment in blasts from acute leukemia patients. When the cut-off point was settled at 13 mV of chemiluminescence all cases of acute myeloid leukemia (AML) were distinguished from those of acute lymphoid leukemia. In addition, this technique was able to demonstrate MPO activity in AML poorly differentiated (FAB-M0) which usually does not stain for MPO in classical cytochemistry preparations and could be negative also by immunocytochemistry with anti-MPO monoclonal antibody. Therefore the method here described presented a higher sensitivity than the immunocytochemistry procedure with anti-MPO.
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Acute leukemia Antimyeloperoxidase Chemiluminescence Myeloperoxidase myeloperoxidase acute granulocytic leukemia acute lymphocytic leukemia adolescent adult aged bone marrow biopsy chemoluminescence child controlled study diagnostic accuracy enzyme activity human human tissue immunocytochemistry infant intermethod comparison major clinical study priority journal Acute Disease Adolescent Adult Aged Aged, 80 and over Antibodies, Monoclonal Child Child, Preschool Comparative Study Diagnosis, Differential Human Immunohistochemistry Immunophenotyping Infant Leukemia, Lymphocytic, Acute Leukemia, Myeloid Middle Age Peroxidase Sensitivity and Specificity Support, Non-U.S. Gov't
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Inglês
Citação
Acta Haematologica, v. 90, n. 1, p. 19-24, 1993.
