Fibronectin induces MMP2 expression in human prostate cancer cells

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dc.contributor.authorMoroz, Andrei[UNESP]
dc.contributor.authorDelella, Flávia K. [UNESP]
dc.contributor.authorLacorte, Lívia M. [UNESP]
dc.contributor.authorDeffune, Elenice [UNESP]
dc.contributor.authorFelisbino, Sérgio L. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:28:13Z
dc.date.available2014-05-27T11:28:13Z
dc.date.issued2013-01-25
dc.description.abstractHigh-grade prostate cancers express high levels of matrix metalloproteinases (MMPs), major enzymes involved in tumor invasion and metastasis. However, the tumor cell lines commonly employed for prostate cancer research express only small amounts of MMPs when cultivated as monolayer cultures, in common culture media. The present study was conducted to ascertain whether culture conditions that include fibronectin can alter MMP2 and MMP9 expression by the human prostatic epithelial cell lines RWPE-1, LNCaP and PC-3. These cells were individually seeded at 2×104cells/cm2, cultivated until they reached 80% confluence, and then exposed for 4h to fibronectin, after which the conditioned medium was analyzed by gelatin zymography. Untreated cells were given common medium. Only RWPE-1 cells express detectable amounts of MMP9 when cultivated in common medium, whereas the addition of fibronectin induced high expression levels of pro and active forms of MMP2 in all tested cell lines. Our findings demonstrate that normal and tumor prostate cell lines express MMP2 activity when in contact with extracellular matrix components or blood plasma proteins such as fibronectin. Future studies of transcriptomes and proteomes in prostate cancer research using these cell lines should not neglect these important conclusions. © 2012 Elsevier Inc..en
dc.description.affiliationUniv Estadual Paulista - UNESP Institute of Biosciences Department of Morphology, Botucatu, SP
dc.description.affiliationUniv Estadual Paulista - UNESP Botucatu Medical School, Blood Transfusion Center, Cell Engineering Lab., Botucatu, SP
dc.description.affiliationUniv Estadual Paulista - UNESP Botucatu Medical School Department of Urology, Botucatu, SP
dc.description.affiliationUnespUniv Estadual Paulista - UNESP Institute of Biosciences Department of Morphology, Botucatu, SP
dc.description.affiliationUnespUniv Estadual Paulista - UNESP Botucatu Medical School, Blood Transfusion Center, Cell Engineering Lab., Botucatu, SP
dc.description.affiliationUnespUniv Estadual Paulista - UNESP Botucatu Medical School Department of Urology, Botucatu, SP
dc.format.extent1319-1321
dc.identifierhttp://dx.doi.org/10.1016/j.bbrc.2012.12.031
dc.identifier.citationBiochemical and Biophysical Research Communications, v. 430, n. 4, p. 1319-1321, 2013.
dc.identifier.doi10.1016/j.bbrc.2012.12.031
dc.identifier.file2-s2.0-84872871834.pdf
dc.identifier.issn0006-291X
dc.identifier.issn1090-2104
dc.identifier.lattes9646764071339214
dc.identifier.scopus2-s2.0-84872871834
dc.identifier.urihttp://hdl.handle.net/11449/74420
dc.identifier.wosWOS:000315075200023
dc.language.isoeng
dc.relation.ispartofBiochemical and Biophysical Research Communications
dc.relation.ispartofjcr2.559
dc.relation.ispartofsjr1,087
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectFibronectin
dc.subjectLNCaP
dc.subjectMMP2
dc.subjectMMP9
dc.subjectPC-3
dc.subjectRWPE-1
dc.subjectfibronectin
dc.subjectgelatin
dc.subjectgelatinase A
dc.subjectplasma protein
dc.subjectcancer cell
dc.subjectcancer cell culture
dc.subjectcell strain LNCaP
dc.subjectcontrolled study
dc.subjectculture medium
dc.subjectenzyme activity
dc.subjectextracellular matrix
dc.subjecthuman
dc.subjecthuman cell
dc.subjectpriority journal
dc.subjectprostate cancer
dc.subjectprostate epithelium
dc.subjectprotein blood level
dc.subjectprotein expression
dc.subjectprotein function
dc.subjectprotein induction
dc.subjectprotein protein interaction
dc.subjectzymography
dc.subjectCell Culture Techniques
dc.subjectCell Line, Tumor
dc.subjectFibronectins
dc.subjectHumans
dc.subjectMale
dc.subjectMatrix Metalloproteinase 2
dc.subjectMatrix Metalloproteinase 9
dc.subjectProstatic Neoplasms
dc.titleFibronectin induces MMP2 expression in human prostate cancer cellsen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
unesp.author.lattes9646764071339214
unesp.author.lattes6926124203948011[1]
unesp.author.orcid0000-0002-4498-9784[1]
unesp.author.orcid0000-0001-6362-5882[2]
unesp.author.orcid0000-0002-0533-3248[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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