Detection of Leishmania spp. using parasitological, serological and molecular assays in asymptomatic and sick cats from an endemic area of visceral leishmaniosis in Brazil

dc.contributor.authorAlves-Martin, Maria Fernanda [UNESP]
dc.contributor.authorPaixão, Mirian dos Santos [UNESP]
dc.contributor.authorda Silva, Diogo Tiago
dc.contributor.authorTenório, Michely da Silva [UNESP]
dc.contributor.authorAlves, Maria Luana
dc.contributor.authorStarke-Buzetti, Wilma Aparecida [UNESP]
dc.contributor.authorPereira, Virginia Bodelão Richini
dc.contributor.authorLucheis, Simone Baldini [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionBauru II Regional Laboratory Center
dc.contributor.institutionAPTA
dc.date.accessioned2018-12-11T17:15:32Z
dc.date.available2018-12-11T17:15:32Z
dc.date.issued2017-11-01
dc.description.abstractObjective: To analyze the prevalence of feline leishmaniosis in 55 asymptomatic cats or assess clinical disease in a visceral leishmaniasis-endemic area using parasitological, serological and molecular techniques. Methods: Fifty-five adult domestic and non-purebred cats held at an animal shelter in a rural area of the municipality of Ilha Solteira, São Paulo, Brazil, a region endemic for canine visceral leishmaniosis, were evaluated. A total of 5 mL of blood was collected from each animal, for blood culture, serological (indirect fluorescent antibody test, soluble extract ELISA, ELISA recombinant K39 antigen) and molecular tests (PCR). At the time of blood collection, the cats underwent clinical evaluation for the presence of clinical signs. Results: Flagellate protozoa were found in nine blood cultures (16.4%). Seropositivity by indirect fluorescent antibody test was observed in 32 serum samples (62.7%). ELISA-SE showed seropositivity in 37 cats (72.5%), while ELISA-rK39 revealed positive results in 11 cats (21.6%). Leishmania spp. was detected by PCR in whole blood samples from five cats (9.1%). Based on clinical evaluation, it was possible to detect the presence of clinical signs (alopecia, emaciation, pinna lesions, nose lesions, skin lesions) in 30 animals (54.5%). Conclusions: To the knowledge of the authors, this is the first report of Leishmania spp. in domestic cats in the epidemiological cycle of leishmaniosis in the studied area, requiring more attention in the establishment of preventive steps to control this disease.en
dc.description.affiliationDepartment of Tropical Diseases Botucatu Medical School São Paulo State University - UNESP
dc.description.affiliationDepartment of Preventive Medicine and Animal Health Faculty of Veterinary Medicine and Animal Husbandry USP-Sao Paulo
dc.description.affiliationDepartment of Microbiology and Immunology São Paulo State University UNESP - Botucatu
dc.description.affiliationDepartment of Biology and Animal Husbandry São Paulo State University UNESP
dc.description.affiliationAdolfo Lutz Institute Bauru II Regional Laboratory Center
dc.description.affiliationPaulista Agency of Agribusiness Technology APTA
dc.description.affiliationUnespDepartment of Tropical Diseases Botucatu Medical School São Paulo State University - UNESP
dc.description.affiliationUnespDepartment of Microbiology and Immunology São Paulo State University UNESP - Botucatu
dc.description.affiliationUnespDepartment of Biology and Animal Husbandry São Paulo State University UNESP
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2010/13602-0
dc.format.extent659-664
dc.identifierhttp://dx.doi.org/10.12980/apjtd.7.2017D7-100
dc.identifier.citationAsian Pacific Journal of Tropical Disease, v. 7, n. 11, p. 659-664, 2017.
dc.identifier.doi10.12980/apjtd.7.2017D7-100
dc.identifier.file2-s2.0-85031920820.pdf
dc.identifier.issn2222-1808
dc.identifier.scopus2-s2.0-85031920820
dc.identifier.urihttp://hdl.handle.net/11449/175369
dc.language.isoeng
dc.relation.ispartofAsian Pacific Journal of Tropical Disease
dc.relation.ispartofsjr0,230
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectBrazil
dc.subjectClinical signs
dc.subjectDiagnostics
dc.subjectDomestic cat
dc.subjectVisceral leishmaniosis
dc.titleDetection of Leishmania spp. using parasitological, serological and molecular assays in asymptomatic and sick cats from an endemic area of visceral leishmaniosis in Brazilen
dc.typeArtigo

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