Breeding protocol for the sand fly Nyssomyia neivai (Diptera: Psychodidae) in laboratory conditions

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Goulart, Thais Marchi
da Rocha Silva, Flávia Benini [UNESP]
Machado, Vicente Estevam [UNESP]
Oliveira, Wanderson Henrique Cruz [UNESP]
de Castro, Camila Feitosa [UNESP]
Rodrigues, Marili Villa Nova
Pinto, Mara Cristina [UNESP]
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The information in this protocol covers from the basic steps and material necessary to start a sand fly colony up to the specific details which are important to the success of a Nyssomyia neivai colony. The greatest problems in our colony of Ny. neivai were solved with specific care, for instance, using vermiculite and an adequate number of adults in oviposition containers; the control of fungus with the exact amount of diet for the larvae and humidity control; a second blood meal for females and control of the number of times animals are used for blood meals. Currently, our colony is at F22 generation.
Colonization, Colony, Life cycle, Phlebotomine, Rearing methods, Sand flies
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Acta Tropica, v. 174, p. 102-105.