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Road-killed wild animals: a preservation problem useful for eco-epidemiological studies of pathogens

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dc.contributor.authorRichini-Pereira, V. B. [UNESP]
dc.contributor.authorBosco, S. M. G. [UNESP]
dc.contributor.authorTheodoro, R. C. [UNESP]
dc.contributor.authorBarrozo, L.
dc.contributor.authorBagagli, Eduardo [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-20T13:39:58Z
dc.date.available2014-05-20T13:39:58Z
dc.date.issued2010-01-01
dc.description.abstractRoad-killed wild animals have been for years used for surveillance of vectors of zoonotic pathogens and may offer new opportunities for eco-epidemiological studies. In the current study, fungal infection was evaluated by PCR and nested-PCR in tissue samples collected from 19 road-killed wild animals. The necropsies were carried out and samples were collected for DNA extraction. Results, using PCR with a panfungal primer and nested PCR with specific primers, indicated that some animals are naturally infected with Amauroascus aureus, Metarhizium anisopliae, Aspergillus flavus, Aspergillus oryzae, Emmonsia parva, Paracoccidioides brasiliensis or Pichia stipitis. The approach employed herein proved useful for detecting the environmental occurrence of several fungi, as well as determining natural reservoirs in wild animals and facilitating the understanding of host-pathogen relationships.en
dc.description.affiliationSão Paulo State Univ UNESP Univ Estadual Paulista, Sch Vet Med & Anim Husb, Dept Vet Hyg & Publ Hlth, Botucatu, SP, Brazil
dc.description.affiliationSão Paulo State Univ UNESP Univ Estadual Paulista, Botucatu Biosci Inst, Dept Microbiol & Immunol, Botucatu, SP, Brazil
dc.description.affiliationUniv São Paulo, Sch Philosophy Literature & Human Sci, Dept Geog, São Paulo, Brazil
dc.description.affiliationUnespSão Paulo State Univ UNESP Univ Estadual Paulista, Sch Vet Med & Anim Husb, Dept Vet Hyg & Publ Hlth, Botucatu, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ UNESP Univ Estadual Paulista, Botucatu Biosci Inst, Dept Microbiol & Immunol, Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 05/56771-9
dc.description.sponsorshipIdFAPESP: 06/03597-4
dc.format.extent607-613
dc.identifierhttp://dx.doi.org/10.1590/S1678-91992010000400011
dc.identifier.citationJournal of Venomous Animals and Toxins Including Tropical Diseases. Botucatu: Cevap-unesp, v. 16, n. 4, p. 607-613, 2010.
dc.identifier.fileS1678-91992010000400011-en.pdf
dc.identifier.issn1678-9199
dc.identifier.lattes3320327570429539
dc.identifier.orcid0000-0002-8003-4109
dc.identifier.scieloS1678-91992010000400011
dc.identifier.urihttp://hdl.handle.net/11449/13877
dc.identifier.wosWOS:000284778000011
dc.language.isoeng
dc.publisherUniversidade Estadual Paulista (Unesp), Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP)
dc.relation.ispartofJournal of Venomous Animals and Toxins Including Tropical Diseases
dc.relation.ispartofjcr1.782
dc.relation.ispartofsjr0,573
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectroad-killed animalsen
dc.subjectfungal pathogensen
dc.subjecteco-epidemiologyen
dc.subjectPCRen
dc.titleRoad-killed wild animals: a preservation problem useful for eco-epidemiological studies of pathogensen
dc.typeArtigo
dcterms.licensehttp://www.scielo.br/revistas/jvatitd/iaboutj.htm
dcterms.rightsHolderCevap-unesp
dspace.entity.typePublication
unesp.author.lattes3320327570429539[5]
unesp.author.orcid0000-0001-7322-6789[4]
unesp.author.orcid0000-0002-8003-4109[5]
unesp.author.orcid0000-0001-8312-7397[1]
unesp.author.orcid0000-0001-5016-1046[3]
unesp.author.orcid0000-0002-6363-3740[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt
unesp.departmentHigiene Veterinária e Saúde Pública - FMVZpt
unesp.departmentMicrobiologia e Imunologia - IBBpt

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Botucatu - FMVZ - Faculdade de Medicina Veterinária e Zootecnia
Botucatu - IBB - Instituto de Biociências