Cytotoxicity of denture base resins: Effect of water bath and microwave postpolymerization heat treatments
dc.contributor.author | Jorge, Janaina Habib [UNESP] | |
dc.contributor.author | Giampaolo, Eunice Teresinha [UNESP] | |
dc.contributor.author | Vergani, Carlos Eduardo [UNESP] | |
dc.contributor.author | Machado, Ana Lucia [UNESP] | |
dc.contributor.author | Pavarina, Ana Claudia [UNESP] | |
dc.contributor.author | Carlos, Iracilda Zeppone [UNESP] | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.date.accessioned | 2014-05-27T11:21:04Z | |
dc.date.available | 2014-05-27T11:21:04Z | |
dc.date.issued | 2004-05-01 | |
dc.description.abstract | Purpose: This study compared the effect of two postpolymerization heat treatments on the cytotoxicity of three denture base resins on L929 cells using 3H-thymidine incorporation and MTT assays. Materials and Methods: Sample disks of Lucitone 550, QC 20, and Acron MC resins were fabricated under aseptic conditions and stored in distilled water at 37°C for 48 hours. Specimens were then divided into three groups: (1) heat treated in microwave oven for 3 minutes at 500 W; (2) heat treated in water bath at 55°C for 60 minutes; and (3) no heat treatment. Eluates were prepared by placing three disks into a sterile glass vial with 9 mL of Eagle's medium and incubating at 37°C for 24 hours. The cytotoxic effect from the eluates was evaluated using the 3H-thymidine incorporation and MTT assays, which reflect DNA synthesis levels and cell metabolism, respectively. Results: The components leached from the resins were cytotoxic to L929 cells when 3H- thymidine incorporation assay was employed. In contrast, eluates from all resins revealed noncytotoxic effects as measured by MTT assay. For both MTT assay and 3H-thymidine incorporation, the heat treatments did not decrease the cytotoxicity of the materials tested. Conclusion: Resins were graded by 3H-thymidine incorporation assay as slightly cytotoxic and by MTT assay as noncytotoxic. Cytotoxicity of the denture base materials was not influenced by microwave or water bath heat treatment. | en |
dc.description.affiliation | Dept. Dent. Mat. and Prosthodontics São Paulo State University Araraquara Dental School, São Paulo | |
dc.description.affiliation | Department of Clinical Analysis São Paulo State University Araraquara Pharmaceutical School, São Paulo | |
dc.description.affiliation | Dept. Dent. Mat. and Prosthodontics Araraquara Dental School, Rua Humaitá 1680, Araraquara, S. Paulo 14801-903 | |
dc.description.affiliationUnesp | Dept. Dent. Mat. and Prosthodontics São Paulo State University Araraquara Dental School, São Paulo | |
dc.description.affiliationUnesp | Department of Clinical Analysis São Paulo State University Araraquara Pharmaceutical School, São Paulo | |
dc.format.extent | 340-344 | |
dc.identifier | http://www.quintpub.com/journals/ijp/abstract.php?article_id=1386 | |
dc.identifier.citation | International Journal of Prosthodontics, v. 17, n. 3, p. 340-344, 2004. | |
dc.identifier.issn | 0893-2174 | |
dc.identifier.lattes | 5737127334248549 | |
dc.identifier.lattes | 3003130522427820 | |
dc.identifier.lattes | 8000248781842587 | |
dc.identifier.lattes | 8867670539105403 | |
dc.identifier.orcid | 0000-0002-7375-4714 | |
dc.identifier.scopus | 2-s2.0-2542514757 | |
dc.identifier.uri | http://hdl.handle.net/11449/67729 | |
dc.identifier.wos | WOS:000221576700015 | |
dc.language.iso | eng | |
dc.relation.ispartof | International Journal of Prosthodontics | |
dc.relation.ispartofjcr | 1.333 | |
dc.relation.ispartofsjr | 0,891 | |
dc.rights.accessRights | Acesso restrito | |
dc.source | Scopus | |
dc.subject | resin | |
dc.subject | thymidine | |
dc.subject | animal cell | |
dc.subject | cell metabolism | |
dc.subject | controlled study | |
dc.subject | culture medium | |
dc.subject | cytotoxicity | |
dc.subject | denture | |
dc.subject | DNA synthesis | |
dc.subject | heat treatment | |
dc.subject | intermethod comparison | |
dc.subject | microwave radiation | |
dc.subject | mouse | |
dc.subject | nonhuman | |
dc.subject | polymerization | |
dc.subject | storage | |
dc.subject | temperature dependence | |
dc.subject | Acrylic Resins | |
dc.subject | Analysis of Variance | |
dc.subject | Animals | |
dc.subject | Coloring Agents | |
dc.subject | Denture Bases | |
dc.subject | Heat | |
dc.subject | L Cells (Cell Line) | |
dc.subject | Mice | |
dc.subject | Mice, Inbred C3H | |
dc.subject | Microwaves | |
dc.subject | Molecular Structure | |
dc.subject | Phase Transition | |
dc.subject | Tetrazolium Salts | |
dc.subject | Tritium | |
dc.subject | Water | |
dc.title | Cytotoxicity of denture base resins: Effect of water bath and microwave postpolymerization heat treatments | en |
dc.type | Artigo | |
dcterms.license | http://www.quintpub.com/terms.php | |
unesp.author.lattes | 5737127334248549 | |
unesp.author.lattes | 3003130522427820[3] | |
unesp.author.lattes | 8000248781842587[4] | |
unesp.author.lattes | 8867670539105403[5] | |
unesp.author.orcid | 0000-0002-7375-4714[3] | |
unesp.author.orcid | 0000-0002-9135-3455[1] | |
unesp.author.orcid | 0000-0002-9231-1994[5] | |
unesp.author.orcid | 0000-0002-2718-428X[4] | |
unesp.campus | Universidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquara | pt |
unesp.campus | Universidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquara | pt |
unesp.department | Análises Clínicas - FCF | pt |
unesp.department | Materiais Odontológicos e Prótese - FOAR | pt |