Cytotoxicity of denture base resins: Effect of water bath and microwave postpolymerization heat treatments

dc.contributor.authorJorge, Janaina Habib [UNESP]
dc.contributor.authorGiampaolo, Eunice Teresinha [UNESP]
dc.contributor.authorVergani, Carlos Eduardo [UNESP]
dc.contributor.authorMachado, Ana Lucia [UNESP]
dc.contributor.authorPavarina, Ana Claudia [UNESP]
dc.contributor.authorCarlos, Iracilda Zeppone [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:21:04Z
dc.date.available2014-05-27T11:21:04Z
dc.date.issued2004-05-01
dc.description.abstractPurpose: This study compared the effect of two postpolymerization heat treatments on the cytotoxicity of three denture base resins on L929 cells using 3H-thymidine incorporation and MTT assays. Materials and Methods: Sample disks of Lucitone 550, QC 20, and Acron MC resins were fabricated under aseptic conditions and stored in distilled water at 37°C for 48 hours. Specimens were then divided into three groups: (1) heat treated in microwave oven for 3 minutes at 500 W; (2) heat treated in water bath at 55°C for 60 minutes; and (3) no heat treatment. Eluates were prepared by placing three disks into a sterile glass vial with 9 mL of Eagle's medium and incubating at 37°C for 24 hours. The cytotoxic effect from the eluates was evaluated using the 3H-thymidine incorporation and MTT assays, which reflect DNA synthesis levels and cell metabolism, respectively. Results: The components leached from the resins were cytotoxic to L929 cells when 3H- thymidine incorporation assay was employed. In contrast, eluates from all resins revealed noncytotoxic effects as measured by MTT assay. For both MTT assay and 3H-thymidine incorporation, the heat treatments did not decrease the cytotoxicity of the materials tested. Conclusion: Resins were graded by 3H-thymidine incorporation assay as slightly cytotoxic and by MTT assay as noncytotoxic. Cytotoxicity of the denture base materials was not influenced by microwave or water bath heat treatment.en
dc.description.affiliationDept. Dent. Mat. and Prosthodontics São Paulo State University Araraquara Dental School, São Paulo
dc.description.affiliationDepartment of Clinical Analysis São Paulo State University Araraquara Pharmaceutical School, São Paulo
dc.description.affiliationDept. Dent. Mat. and Prosthodontics Araraquara Dental School, Rua Humaitá 1680, Araraquara, S. Paulo 14801-903
dc.description.affiliationUnespDept. Dent. Mat. and Prosthodontics São Paulo State University Araraquara Dental School, São Paulo
dc.description.affiliationUnespDepartment of Clinical Analysis São Paulo State University Araraquara Pharmaceutical School, São Paulo
dc.format.extent340-344
dc.identifierhttp://www.quintpub.com/journals/ijp/abstract.php?article_id=1386
dc.identifier.citationInternational Journal of Prosthodontics, v. 17, n. 3, p. 340-344, 2004.
dc.identifier.issn0893-2174
dc.identifier.lattes5737127334248549
dc.identifier.lattes3003130522427820
dc.identifier.lattes8000248781842587
dc.identifier.lattes8867670539105403
dc.identifier.orcid0000-0002-7375-4714
dc.identifier.scopus2-s2.0-2542514757
dc.identifier.urihttp://hdl.handle.net/11449/67729
dc.identifier.wosWOS:000221576700015
dc.language.isoeng
dc.relation.ispartofInternational Journal of Prosthodontics
dc.relation.ispartofjcr1.333
dc.relation.ispartofsjr0,891
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectresin
dc.subjectthymidine
dc.subjectanimal cell
dc.subjectcell metabolism
dc.subjectcontrolled study
dc.subjectculture medium
dc.subjectcytotoxicity
dc.subjectdenture
dc.subjectDNA synthesis
dc.subjectheat treatment
dc.subjectintermethod comparison
dc.subjectmicrowave radiation
dc.subjectmouse
dc.subjectnonhuman
dc.subjectpolymerization
dc.subjectstorage
dc.subjecttemperature dependence
dc.subjectAcrylic Resins
dc.subjectAnalysis of Variance
dc.subjectAnimals
dc.subjectColoring Agents
dc.subjectDenture Bases
dc.subjectHeat
dc.subjectL Cells (Cell Line)
dc.subjectMice
dc.subjectMice, Inbred C3H
dc.subjectMicrowaves
dc.subjectMolecular Structure
dc.subjectPhase Transition
dc.subjectTetrazolium Salts
dc.subjectTritium
dc.subjectWater
dc.titleCytotoxicity of denture base resins: Effect of water bath and microwave postpolymerization heat treatmentsen
dc.typeArtigo
dcterms.licensehttp://www.quintpub.com/terms.php
unesp.author.lattes5737127334248549
unesp.author.lattes3003130522427820[3]
unesp.author.lattes8000248781842587[4]
unesp.author.lattes8867670539105403[5]
unesp.author.orcid0000-0002-7375-4714[3]
unesp.author.orcid0000-0002-9135-3455[1]
unesp.author.orcid0000-0002-9231-1994[5]
unesp.author.orcid0000-0002-2718-428X[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt

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