Multiple nucleolus organizer regions in Leptodactylus mystacinus (Amphibia, Anura) and comments on its systematic position in the L fuscus group based on cytogenetic and molecular analyses

dc.contributor.authorZampieri Silva, Ana Paula
dc.contributor.authorBaptista Haddad, Celio Fernando
dc.contributor.authorGalassi, Guilherme Guidolin
dc.contributor.authorKasahara, Sanae
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-02-26T17:25:39Z
dc.date.accessioned2014-05-20T13:59:04Z
dc.date.available2014-02-26T17:25:39Z
dc.date.available2014-05-20T13:59:04Z
dc.date.issued2006-05-01
dc.description.abstractSpecimens of Leptodactylus mystacinus from Brazil were karyotyped with conventional and differential staining. The 2n = 22 karyotype is similar to that found for the majority of the Leptodactylus, the karyotypic conservatism also confirmed by the similarity of the replication banding patterns with those previously described. L. mystacinus has a small amount of C-banded heterochromatin, located mainly at the centromeres, although telomeric or interstitial bands have also been noticed. With DA/CMA(3) some chromosome regions showed slightly bright fluorescence, and with DA/DAPI, no particular AT-rich repetitive region was observed. Silver staining showed an extensive inter- and intraindividual variation in the number and position of Ag-positive regions, in 1p, 4p, 8p, 8q, and 11p. Nevertheless, FISH using rDNA probes confirmed only the signals on the short arms of chromosomes 4 and 8 as true NORs. The remaining silver stained regions are probably due to the heterochromatin with some affinity to the Ag-staining. Phylogenetic analysis based on partial cytochrome b sequence revealed that L. mystacinus forms a basal branch, so that the presence of multiple NORs in pairs 4 and 8 in this species indicates an autapomorphy.en
dc.description.affiliationUNESP, Dept Biol, Inst Biociencias, BR-13506900 Rio Claro, SP, Brazil
dc.description.affiliationUNESP, Dept Zool, Inst Biociencias, BR-13506900 Rio Claro, SP, Brazil
dc.description.affiliationUnespUNESP, Dept Biol, Inst Biociencias, BR-13506900 Rio Claro, SP, Brazil
dc.description.affiliationUnespUNESP, Dept Zool, Inst Biociencias, BR-13506900 Rio Claro, SP, Brazil
dc.format.extent35-44
dc.identifierhttp://dx.doi.org/10.1007/s10709-005-2450-z
dc.identifier.citationGenetica. Dordrecht: Springer, v. 127, n. 1-3, p. 35-44, 2006.
dc.identifier.doi10.1007/s10709-005-2450-z
dc.identifier.issn0016-6707
dc.identifier.lattes0458077399058762
dc.identifier.lattes8422327495725206
dc.identifier.urihttp://hdl.handle.net/11449/20989
dc.identifier.wosWOS:000239164900004
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofGenetica
dc.relation.ispartofjcr1.366
dc.relation.ispartofsjr0,649
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectAg-NORspt
dc.subjectBrdU incorporationpt
dc.subjectC-bandingpt
dc.subjectFISHpt
dc.subjectfluorochrome stainingpt
dc.titleMultiple nucleolus organizer regions in Leptodactylus mystacinus (Amphibia, Anura) and comments on its systematic position in the L fuscus group based on cytogenetic and molecular analysesen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer
unesp.author.lattes0458077399058762[2]
unesp.author.lattes8422327495725206
unesp.author.orcid0000-0002-7044-5764[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claropt

Arquivos

Licença do Pacote
Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição: