Sex determination of murine and bovine embryos using cytotoxicity and immunofluorescence assays
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Murine and bovine embryos were sexed by using polyclonal and monoclonal anti-H-Y antibodies in cytotoxic and immunofluorescence assays. The specificity of 3 different sources of antibodies are compared. Sexing was accomplished by complement-mediated cytotoxicity or by H-Y antigen detection using a anti-mouse Ig fluorescein conjugate. Cytogenetic ascertainment for correct sex identification yielded a score of about 85% in both species, while the immunofluorescence assay yielded a score of 83%. © 1993.
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anti-H-Y antibodies, embryo, immunological assays, sexing
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Inglês
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Theriogenology, v. 39, n. 6, p. 1343-1352, 1993.
