Inhibitory Effect of Gallic Acid and Its Esters on 2,2 '-Azobis(2-amidinopropane)hydrochloride (AAPH)-Induced Hemolysis and Depletion of Intracellular Glutathione in Erythrocytes

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dc.contributor.authorXimenes, Valdecir F. [UNESP]
dc.contributor.authorLopes, Mariana G. [UNESP]
dc.contributor.authorPetronio, Maicon Segalla [UNESP]
dc.contributor.authorRegasini, Luis Octavio [UNESP]
dc.contributor.authorSiqueira Silva, Dulce H. [UNESP]
dc.contributor.authorda Fonseca, Luis M. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:23:53Z
dc.date.available2014-05-20T13:23:53Z
dc.date.issued2010-05-12
dc.description.abstractThe protective effect of gallic acid and its esters, methyl, propyl, and lauryl gallate, against 2,2'-azobis(2-amidinopropane)hydrochloride (AAPH)-induced hemolysis and depletion of intracellular glutathione (GSH) in erythrocytes was studied. The inhibition of hemolysis was dose-dependent, and the esters were significantly more effective than gallic acid. Gallic acid and its esters were compared with regard to their reactivity to free radicals, using the DPPH and AAPH/pyranine free-cell assays, and no significant difference was obtained. Gallic acid and its esters not only failed to inhibit the depletion of intracellular GSH in erythrocytes induced by AAPH but exacerbated it. Similarly, the oxidation of GSH by AAPH or horseradish peroxidase/H(2)O(2) in cell-free systems was exacerbated by gallic acid or gallates. This property could be involved in the recent findings on proapoptotic and pro-oxidant activities of gallates in tumor cells. We provide evidence that lipophilicity and not only radical scavenger potency is an important factor regarding the efficiency of antihemolytic substances.en
dc.description.affiliationUniv Estadual Paulista, UNESP, Fac Ciencias, Dept Quim, BR-17033360 Bauru, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, UNESP, Fac Ciencias Farmaceut, Dept Anal Clin, Araraquara, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, UNESP, Inst Quim, Dept Quim Organ, Araraquara, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, UNESP, Fac Ciencias, Dept Quim, BR-17033360 Bauru, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, UNESP, Fac Ciencias Farmaceut, Dept Anal Clin, Araraquara, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, UNESP, Inst Quim, Dept Quim Organ, Araraquara, SP, Brazil
dc.format.extent5355-5362
dc.identifierhttp://dx.doi.org/10.1021/jf100233y
dc.identifier.citationJournal of Agricultural and Food Chemistry. Washington: Amer Chemical Soc, v. 58, n. 9, p. 5355-5362, 2010.
dc.identifier.doi10.1021/jf100233y
dc.identifier.issn0021-8561
dc.identifier.urihttp://hdl.handle.net/11449/7286
dc.identifier.wosWOS:000277237500021
dc.language.isoeng
dc.publisherAmer Chemical Soc
dc.relation.ispartofJournal of Agricultural and Food Chemistry
dc.relation.ispartofjcr3.412
dc.relation.ispartofsjr1,269
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectGallic aciden
dc.subjectgallatesen
dc.subjecterythrocytesen
dc.subjecthemolysisen
dc.subjectglutathioneen
dc.subjectperoxyl radicalen
dc.subjectantioxidanten
dc.subjectpro-oxidant activityen
dc.titleInhibitory Effect of Gallic Acid and Its Esters on 2,2 '-Azobis(2-amidinopropane)hydrochloride (AAPH)-Induced Hemolysis and Depletion of Intracellular Glutathione in Erythrocytesen
dc.typeArtigo
dcterms.licensehttp://pubs.acs.org/page/policy/nih/index.html
dcterms.rightsHolderAmer Chemical Soc
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Química, Araraquarapt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências, Baurupt
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