Protein profiles identified by LC-MS/MS demonstrate change in beta oxidation, ketogenesis, and propionate metabolism in rumen epithelium with different additives

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dc.contributor.authorRocha, Leone Campos [UNESP]
dc.contributor.authorAssunção, Andrey Sávio de Almeida [UNESP]
dc.contributor.authorMartins, Renata Aparecida [UNESP]
dc.contributor.authorCarvalho, Victor Valério de
dc.contributor.authorPerdigão, Alexandre
dc.contributor.authorBuzalaf, Marília Afonso Rabelo
dc.contributor.authorAdamec, Jiri
dc.contributor.authorBraga, Camila Pereira
dc.contributor.authorMillen, Danilo Domingues [UNESP]
dc.contributor.authorVieira, José Cavalcante Souza [UNESP]
dc.contributor.authorPadilha, Pedro de Magalhães [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionDSM Nutritional Products SA
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversity of Nebraska (UNL)
dc.date.accessioned2023-07-29T16:16:08Z
dc.date.available2023-07-29T16:16:08Z
dc.date.issued2023-08-01
dc.description.abstractReplacement of ionophore feed additives by essential oils demonstrates differences in energy metabolism. The mechanism of action of essential oils depends on the ruminal pH, therefore, feedlot diets with high starch inclusions tend to show greater effectiveness of these natural additives. The aim of this study is to map the proteome of the ruminal epithelium of feedlot Nellore cattle (n = 60) with different additives (monensin, blend of essential oils + exogenous α-amylase) and varying levels of starch (25% and 45%) Two-dimensional polyacrylamide gel electrophoresis was used to separate the proteome of ruminal epithelium. The differentially expressed protein spots were characterized by isoelectric point, molecular mass, volume, and intensity of the spots and identified by liquid chromatography tandem mass spectrometry (LC-MS/MS). Diets using an essential oil blend associated with exogenous amylase promoted greater expression of macromolecules from the degradation of carbohydrates through the glycolytic and ketogenesis pathway. Fourteen proteins were identified in upregulation and the presence of proteins involved in glucose oxidation as well as Hydroxymethylglutaryl–CoA lyase (HMGCL), which catalyzes part of the metabolic intermediate metabolism—a key step in ketogenesis. Our results suggest that there was an increase in glycolysis from the oxidation of Glyceraldehyde-3-phosphate dehydrogenase (GAPDH GAPD), which participates in the first step of acetate and butyrate production and the oxidative decarboxylation of the ruminal epithelium of feedlot Nellore cattle. Monensin increased precursors of propionate, such as methylmalonyl-CoA mutase (MUT), which suggests greater synthesis of propionate via propionyl-CoA upon entry into the citric acid cycle as succinyl-CoA, which can increase feed efficiency and reduces feed intake.en
dc.description.affiliationSão Paulo State University (UNESP) School of Veterinary Medicine and Animal Science, São Paulo
dc.description.affiliationDSM Nutritional Products SA
dc.description.affiliationUniversity of São Paulo (USP) Bauru School of Dentistry, São Paulo
dc.description.affiliationUniversity of Nebraska (UNL) Department of Biochemistry
dc.description.affiliationSão Paulo State University UNESP College of Technology and Agricultural Sciences, São Paulo
dc.description.affiliationSão Paulo State University (UNESP) Institute of Biosciences, São Paulo
dc.description.affiliationUnespSão Paulo State University (UNESP) School of Veterinary Medicine and Animal Science, São Paulo
dc.description.affiliationUnespSão Paulo State University UNESP College of Technology and Agricultural Sciences, São Paulo
dc.description.affiliationUnespSão Paulo State University (UNESP) Institute of Biosciences, São Paulo
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdCAPES: 001
dc.description.sponsorshipIdCAPES: 88881.3107432018–0.1
dc.description.sponsorshipIdFAPESP: FAPESP 19/02538–4
dc.identifierhttp://dx.doi.org/10.1016/j.livsci.2023.105269
dc.identifier.citationLivestock Science, v. 274.
dc.identifier.doi10.1016/j.livsci.2023.105269
dc.identifier.issn1871-1413
dc.identifier.scopus2-s2.0-85161309720
dc.identifier.urihttp://hdl.handle.net/11449/250039
dc.language.isoeng
dc.relation.ispartofLivestock Science
dc.sourceScopus
dc.subjectEnergy metabolism
dc.subjectMass spectrometry
dc.subjectMonensin
dc.subjectPropionate
dc.titleProtein profiles identified by LC-MS/MS demonstrate change in beta oxidation, ketogenesis, and propionate metabolism in rumen epithelium with different additivesen
dc.typeArtigo
unesp.author.orcid0000-0003-3715-0312[1]
unesp.author.orcid0000-0001-7681-1983[3]
unesp.author.orcid0000-0002-1253-7310[9]
unesp.departmentZootecnia - FCATpt

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Dracena - FCAT - Faculdade de Ciências Agrárias e Tecnológicas
Artigos - Química e Bioquímica - IBB