Pro-oxidant activity of apocynin radical

dc.contributor.authorGomes Castor, Lidyane Regina [UNESP]
dc.contributor.authorLocatelli, Katia Andreza [UNESP]
dc.contributor.authorXimenes, Valdecir Farias [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:49:13Z
dc.date.available2014-05-20T13:49:13Z
dc.date.issued2010-06-15
dc.description.abstractApocynin has been widely used as an NADPH oxidase inhibitor in many experimental models. However, concern regarding the efficacy, selectivity, and oxidative side effects of the inhibitor is increasing. In this study, our aim was to characterize the pro-oxidant properties of apocynin and the structurally-related compounds vanillin and vanillic acid. Glutathione (GSH), cysteine, ovalbumin, and the coenzyme NADPH were chosen as potential target biomolecules that could be affected by transient free radicals from apocynin, vanillin and vanillic acid. Additionally, trolox and rifampicin were used as models of hydroquinone moieties, which are particularly susceptible to oxidation. Transient radicals were generated by horseradish peroxidase/hydrogen peroxide-mediated oxidation. In the presence of apocynin, oxidation of GSH was increased seven-fold, and the product of this reaction was identified as GSSG. Similar results were obtained for oxidation of cysteine and ovalbumin. Oxidation of the coenzyme NADPH increased more than 100-fold in the presence of apocynin. Apocynin also caused rapid oxidation of trolox and rifampicin to their quinone derivatives. In conclusion, the pro-oxidant activity of apocynin is related to its previous oxidation leading to transient free radicals. This characteristic may underlie some of the recent findings regarding beneficial or deleterious effects of the phytochemical. (C) 2010 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniv Estadual Paulista, Fac Ciencias Bauru, Dept Quim, BR-17033360 São Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista, Inst Biociencias Botucatu, Dept Farmacol, BR-17033360 São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Ciencias Bauru, Dept Quim, BR-17033360 São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Biociencias Botucatu, Dept Farmacol, BR-17033360 São Paulo, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent1636-1643
dc.identifierhttp://dx.doi.org/10.1016/j.freeradbiomed.2010.03.010
dc.identifier.citationFree Radical Biology and Medicine. New York: Elsevier B.V., v. 48, n. 12, p. 1636-1643, 2010.
dc.identifier.doi10.1016/j.freeradbiomed.2010.03.010
dc.identifier.issn0891-5849
dc.identifier.lattes4066413997908572
dc.identifier.urihttp://hdl.handle.net/11449/17527
dc.identifier.wosWOS:000278043000009
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofFree Radical Biology and Medicine
dc.relation.ispartofjcr6.020
dc.relation.ispartofsjr2,178
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectApocyninen
dc.subjectVanillinen
dc.subjectVanillic aciden
dc.subjectPro-oxidant activityen
dc.subjectGlutathioneen
dc.subjectQuinoneen
dc.subjectNADPH oxidaseen
dc.titlePro-oxidant activity of apocynin radicalen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.orcid0000-0003-2636-3080[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências, Baurupt
unesp.departmentQuímica - FCpt

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