Progesterone upregulates GATA-1 on erythroid progenitors cells in liquid culture

Nenhuma Miniatura disponível




Duarte, ADS
Sales, TSI
Mengel, J. O.
Costa, F. F.
Saad, STO

Título da Revista

ISSN da Revista

Título de Volume


Elsevier B.V.


Steroids hormones modify the hematological features of homozygous sickle cell disease, including the levels of fetal hemoglobin. We used semi-quantitative RT-PCR analysis of GATA-1, GATA-2, NF-E2, and gamma-globin mRNA levels in a two-phase liquid culture system of human adult erythroid cells in order to assay the effect of progesterone upon gene expression. The levels of expression of GATA-1 and gamma-globin mRNA were significantly increased in cells treated with progesterone compared to untreated cells (1.7- to 2.0-fold). Progesterone treatment did not produce any stimulatory effect upon GATA-2 and NF-E2 mRNA expression. Differences in the synthesis of HbF protein could not be detected by flow cytometry, although we observed a small difference in mean intensity fluorescence between cells treated and cells untreated with progesterone on days 7 and 9. Using anti-transferrin receptor and anti-glycophorin A antibodies, we verified that addition of progesterone did not cause any change in erythroid proliferation and differentiation. In conclusion, it is possible that the increased expression of gamma-globin mRNA after progesterone treatment observed in this study may be related to the increased GATA-1 mRNA expression. Interactions of the steroid receptors with the basal transcriptional machinery and with transcription factors might mediate their transcriptional effects. (C) 2002 Elsevier B.V. (USA).



HbF, progesterone, transcription factors, RT-PCR, gene expression

Como citar

Blood Cells Molecules and Diseases. San Diego: Academic Press Inc. Elsevier B.V., v. 29, n. 2, p. 213-224, 2002.