Sodium intake, brain c-Fos protein and gastric emptying in cell-dehydrated rats treated with methysergide into the lateral parabrachial nucleus

dc.contributor.authorDavid, Richard B. [UNESP]
dc.contributor.authorRoncari, Camila F. [UNESP]
dc.contributor.authorLauar, Mariana R. [UNESP]
dc.contributor.authorVendramini, Regina C. [UNESP]
dc.contributor.authorAntunes-Rodrigues, José
dc.contributor.authorMenani, José V. [UNESP]
dc.contributor.authorLuca, Laurival A. de [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2015-12-07T15:40:27Z
dc.date.available2015-12-07T15:40:27Z
dc.date.issued2015-11-01
dc.description.abstractPrevious studies from our laboratory have shown that methysergide, a serotonergic antagonist, injected into the lateral parabrachial nucleus (LPBN) combined with a pre-load of 2 M NaCl, given by gavage, induces 0.3 M NaCl intake. The mechanisms involved in this paradoxical behavior are still unknown. In the present work, we investigated the effect of serotonergic blockade into the LPBN on hindbrain and hypothalamic activity, gastric emptying and arterial blood pressure in cell-dehydrated rats. Methysergide plus 2 M NaCl infused intragastrically or intravenously promoted 0.3 M NaCl intake in two-bottle tests. In cell-dehydrated rats with no access to fluids, methysergide compared to vehicle increased Fos immunoreactivity in the medial nucleus of the solitary tract, area postrema and non-oxytocinergic cells of the ventral portion of the hypothalamic paraventricular nucleus (PVN). There was no alteration in the number of neurons double-labeled for Fos-ir and oxytocin in the PVN and supraoptic nuclei. There was also no alteration in plasma oxytocin and vasopressin, or arterial pressure. In rats cell-dehydrated by i.v. 2 M NaCl, methysergide also did not change the amount of an intragastric load of 0.3 M NaCl retained in the stomach or intestine. The results suggest that methysergide injected into the LPBN of cell-dehydrated rat does not alter primary inhibitory signals that control sodium intake. The inhibitory signals blocked by methysergide in the LPBN possibly originated from activation of brain osmoreceptors, second order visceral/hormonal signals or a combination of both.en
dc.description.affiliationDepartment of Physiology and Pathology, School of Dentistry, São Paulo State University, UNESP, Araraquara, São Paulo, Brazil.
dc.description.affiliationDepartment of Clinical Analysis, School of Pharmaceutical Sciences, São Paulo State University, UNESP, Araraquara, São Paulo, Brazil.
dc.description.affiliationDepartment of Physiology, School of Medicine of Ribeirão Preto, USP, Ribeirão Preto, São Paulo, Brazil.
dc.description.affiliationUnespDepartment of Physiology and Pathology, School of Dentistry, São Paulo State University, UNESP, Araraquara, São Paulo, Brazil.
dc.description.affiliationUnespDepartment of Clinical Analysis, School of Pharmaceutical Sciences, São Paulo State University, UNESP, Araraquara, São Paulo, Brazil.
dc.description.affiliationUnespDepartment of Physiology and Pathology, School of Dentistry, São Paulo State University, UNESP, Araraquara, São Paulo, Brazil. Electronic address: lucajr@foar.unesp.br.
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdCNPq: 301296/2009-0
dc.description.sponsorshipIdFAPESP-PRONEX: 2011/50770-1
dc.description.sponsorshipIdFAPESP: 2007/54523-3
dc.description.sponsorshipIdFAPESP: 2010/20407-0
dc.description.sponsorshipIdFAPESP: 2013/05189-4
dc.format.extent111-120
dc.identifierhttp://dx.doi.org/10.1016/j.physbeh.2015.07.014
dc.identifier.citationPhysiology & Behavior, v. 151, p. 111-120, 2015.
dc.identifier.doi10.1016/j.physbeh.2015.07.014
dc.identifier.issn1873-507X
dc.identifier.pubmed26171591
dc.identifier.urihttp://hdl.handle.net/11449/131681
dc.language.isoeng
dc.publisherElsevier B. V.
dc.relation.ispartofPhysiology & Behavior
dc.rights.accessRightsAcesso restrito
dc.sourcePubMed
dc.subjectDehydrationen
dc.subjectMedulla oblongataen
dc.subjectNeurohypophysisen
dc.subjectSatietyen
dc.subjectSodium appetiteen
dc.subjectThirsten
dc.titleSodium intake, brain c-Fos protein and gastric emptying in cell-dehydrated rats treated with methysergide into the lateral parabrachial nucleusen
dc.typeArtigo
dcterms.rightsHolderElsevier B. V.
unesp.author.orcid0000-0001-8270-2652[7]
unesp.author.orcid0000-0003-1167-4441[6]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt

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