Expression of genes related to quality of Longissimus dorsi muscle meat in Nellore (Bos indicus) and Canchim (5/8 Bos taurus×3/8 Bos indicus) cattle

dc.contributor.authorGiusti, Juliana [UNESP]
dc.contributor.authorCastan, Eduardo [UNESP]
dc.contributor.authorDal Pai, Maeli [UNESP]
dc.contributor.authorArrigoni, Mario de Beni [UNESP]
dc.contributor.authorRodrigues Baldin, Samira [UNESP]
dc.contributor.authorDe Oliveira, Henrique Nunes [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:29:35Z
dc.date.available2014-05-27T11:29:35Z
dc.date.issued2013-06-01
dc.description.abstractThis study was performed to compare CAPN1, CAPN2, CAST, TG, DGAT1 and LEP gene expressions and correlate them with meat quality traits in two genetic groups (Nellore and Canchim) in order to assess their expression profile and use their expression profile as genetic markers. We analyzed 30 young bulls (1. year old), 15 of each genetic group. Samples of the Longissimus dorsi muscle were collected for analysis of: total lipids (TL) and meat tenderness measured as Warner-Bratzler shear force (SF) and myofibrillar fragmentation (MFI) at day of slaughter and 7. days of aging. Gene expression profiles were obtained via RT-qPCR. TL and MFI showed differences between breeds, higher MFI in Canchim and higher TL in Nellore. Calpains showed no differential expression between groups, as did DGAT1, TG, and LEP. CAST was expressed more in the Nellore cattle. The only significant within-breed correlation (0.79) between gene expression and meat traits was found for DGAT1 and MFI in Canchim breed. Although the number of animals used in this study was small, the results indicate that the increased expression of CAST in Nellore may reflect tougher meat, but the lack of correlations with the meat traits indicates it is not a promising genetic marker. © 2013 Elsevier Ltd.en
dc.description.affiliationDepartamento de Zootecnia Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista, Jaboticabal, SP 14884-900
dc.description.affiliationDepartamento de Morfologia Instituto de Biociências Universidade Estadual Paulista, Botucatu, SP 18618-000
dc.description.affiliationDepartamento de Melhoramento e Nutrição Animal Faculdade de Medicina Veterinária e Zootecnia Universidade Estadual Paulista, Botucatu, SP 18618-000
dc.description.affiliationUnespDepartamento de Zootecnia Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista, Jaboticabal, SP 14884-900
dc.description.affiliationUnespDepartamento de Morfologia Instituto de Biociências Universidade Estadual Paulista, Botucatu, SP 18618-000
dc.description.affiliationUnespDepartamento de Melhoramento e Nutrição Animal Faculdade de Medicina Veterinária e Zootecnia Universidade Estadual Paulista, Botucatu, SP 18618-000
dc.format.extent247-252
dc.identifierhttp://dx.doi.org/10.1016/j.meatsci.2013.02.006
dc.identifier.citationMeat Science, v. 94, n. 2, p. 247-252, 2013.
dc.identifier.doi10.1016/j.meatsci.2013.02.006
dc.identifier.file2-s2.0-84875245992.pdf
dc.identifier.issn0309-1740
dc.identifier.lattes5593441035110683
dc.identifier.lattes0555360557852992
dc.identifier.scopus2-s2.0-84875245992
dc.identifier.urihttp://hdl.handle.net/11449/75521
dc.identifier.wosWOS:000317450100015
dc.language.isoeng
dc.relation.ispartofMeat Science
dc.relation.ispartofjcr2.821
dc.relation.ispartofsjr1,643
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectFat
dc.subjectProteolytic enzymes
dc.subjectRT-qPCR
dc.subjectDifferential expressions
dc.subjectExpression profile
dc.subjectGene expression profiles
dc.subjectGenetic markers
dc.subjectLongissimus dorsi
dc.subjectProteolytic enzyme
dc.subjectMeats
dc.subjectMuscle
dc.subjectPolymerase chain reaction
dc.subjectGene expression
dc.subjectanimal
dc.subjectcattle
dc.subjectcross breeding
dc.subjectgene expression regulation
dc.subjectgenetics
dc.subjectmeat
dc.subjectphysiology
dc.subjectskeletal muscle
dc.subjectstandard
dc.subjectAnimals
dc.subjectCattle
dc.subjectCrosses, Genetic
dc.subjectGene Expression Regulation
dc.subjectMeat
dc.subjectMuscle, Skeletal
dc.titleExpression of genes related to quality of Longissimus dorsi muscle meat in Nellore (Bos indicus) and Canchim (5/8 Bos taurus×3/8 Bos indicus) cattleen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
unesp.author.lattes5593441035110683
unesp.author.lattes0555360557852992
unesp.author.orcid0000-0002-3181-1223[6]
unesp.author.orcid0000-0003-3224-1688[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt
unesp.departmentZootecnia - FCAVpt

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