Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building
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Int Union Against Tuberculosis Lung Disease (i U A T L D)
International Union Against Tuberculosis and Lung Disease
International Union Against Tuberculosis and Lung Disease
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Resumo
The diagnosis of tuberculosis is seriously hampered in the absence of standard biosafety laboratory facilities for specimen concentration and Mycobacterium tuberculosis culture. Within a laboratory twinning arrangement, heat-fixed direct smear and sediment from 74 bleach-processed and 20 non-processed specimens from Cumura Hospital, Guinea-Bissau, were sent to Lisbon for molecular evaluation of rifampicin resistance. Sequence analysis of a 369 base-pair ppoB locus detected 3.2% (3/94) resistant specimens. To our knowledge, this represents the first report on the molecular analysis of M. tuberculosis from bleach-processed sputum, an alternative to current diagnostic practice in low-resource settings.
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International Journal of Tuberculosis and Lung Disease. Paris: Int Union Against Tuberculosis Lung Disease (i U A T L D), v. 16, n. 11, p. 1535-1537, 2012.
