Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex
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2014-09-01Type
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We evaluated a multiplex-PCR to differentiate Mycobacterium bovis from M. tuberculosis Complex (MTC) by one step amplification based on simultaneous detection of pncA 169C > G change in M. bovis and the IS6110 present in MTC species. Our findings showed the proposed multiplex-PCR is a very useful tool for complementation in differentiating M. bovis from other cultured MTC species.
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Spositto, F.l.e. et al. Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex. Brazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 45, n. 3, p. 841-843, 2014. Available at: <http://hdl.handle.net/11449/114347>.
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