Standartization of broth microdilution method for Mycobacterium tuberculosis
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Data
2000-01-01
Autores
Leite, Clarice Queico Fujimura [UNESP]
Beretta, Ana Laura Remédio Zeni [UNESP]
Anno, Ivone Shizuko [UNESP]
Telles, Maria Alice da Silva
Título da Revista
ISSN da Revista
Título de Volume
Editor
Instituto Oswaldo Cruz, Ministério da Saúde
Resumo
Indirect drug susceptibility tests of Mycobacterium tuberculosis was done to investigate the accuracy and feasibility of a broth microdilution method (BMM) for determining minimal inhibitory concentrations of conventional drugs against M. tuberculosis. Test drugs included isoniazid (H), rifampicin (R), ethambutol (E), streptomycin (S) and pyrazinamide (Z). Fifty isolates of M. tuberculosis from patients who had never received drug therapy, and H37Rv strain for control, were evaluated in the system. When comparing this method with the gold standard proportional method in Lowenstein-Jensen medium, sensitivity of 100% for all drugs and specifities of 91, 100, 96, 98 and 85% were observed respectively for H, R, E, S and Z. The BMM was read faster (14-20 days) than the proportional method (20-28 days). The microdilution method evaluated allows the testing of multiple drugs in multiple concentrations. It is easy to perform and does not require special equipment or expensive supplies. In contrast to radiometric method it does not use radioactive material.
Descrição
Palavras-chave
broth microdilution method, Mycobacterium tuberculosis, drugs susceptibility test
Como citar
Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 95, n. 1, p. 127-129, 2000.