Comparison of three molecular typing methods to assess genetic diversity for Mycobacterium tuberculosis
Carregando...
Fontes externas
Fontes externas
Data
Orientador
Coorientador
Pós-graduação
Curso de graduação
Título da Revista
ISSN da Revista
Título de Volume
Editor
Elsevier
Tipo
Artigo
Direito de acesso
Acesso aberto
Fontes externas
Fontes externas
Resumo
This study describes the comparison of three methods for genotyping of Mycobacterium tuberculosis, namely MIRU-VNTR (mycobacterial interspersed repetitive units-variable number of tandem repeats), spoligotyping and, for the first time, MLST (Multilocus Sequence Typing). In order to evaluate the discriminatory power of these methods, a total of 44 M. tuberculosis isolates obtained from sputum specimens of patients from Brazil were genotyped. Among the three methods, MLST showed the lowest discriminatory power compared to the other two techniques. MIRU-VNTR showed better discriminatory power when compared to spoligotyping, however, the combination of both methods provides the greatest level of discrimination and therefore this combination is the most useful genotyping tool to be applied to M. tuberculosis isolates. © 2013 Elsevier B.V.
Descrição
Palavras-chave
M. tuberculosis MIRU-VNTR MLST Spoligotyping adult bacterial genome bacterial strain bacterium identification bacterium isolate Brazil cladistics controlled study gene cluster genetic variability human intermethod comparison molecular model molecular typing multilocus sequence typing Mycobacterium tuberculosis nonhuman nucleotide sequence priority journal sequence analysis variable number of tandem repeat Adult Aged Aged, 80 and over Female Genetic Variation Humans Male Middle Aged Molecular Epidemiology Molecular Typing Sensitivity and Specificity Sputum Tuberculosis, Pulmonary Young Adult
Idioma
Inglês
Citação
Journal of Microbiological Methods, v. 93, n. 1, p. 42-48, 2013.
