Validation of an alternative analytical method for the quantification of antioxidant activity in plant extracts
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Data
2013-04-15
Autores
Almeida, Maria G. J.
Chiari, Bruna G.
Correa, Marcos Antonio [UNESP]
Chin, Chung Man [UNESP]
Isaac, Vera Lucia Borges [UNESP]
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Resumo
SUMMARY. A new method was developed to evaluate the total antioxidant activity in plant extracts, which was based on radical scavenging using 2,2'-azinobis-(3-ethylbenzothiazoline)-6-sulfonic acid. The proposed method was linear in the range of 0.86 to 26.0 μg/mL, displaying a correlation coefficient of 0.999. Moreover, the accuracy and precision analysis showed agreement with ANVISA guidelines (96.66 to 98.46% and < 5.0%, respectively). The method demonstrated sensitivity, robustness and efficiency in detecting low concentrations of plant extracts (detection and quantification limit of 0.86 and 2.87 μg/mL, respectively) that may be present in pharmaceuticals and cosmetics preparations, again in agreement with ANVISA. In addition, the assay is inexpensive and easy to perform.
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Antioxidant activity, Plant extracts, Spectrophotometry UV-Vis, 2,2' azinobis(3 ethylbenzothiazoline 6 sulfonic acid), antioxidant, Ascophyllum nodosum extract, plant extract, unclassified drug, accuracy, antioxidant activity, Ascophyllum, ascophyllum nodosum, drug determination, drug screening, drug stability, instrument validation, limit of detection, limit of quantitation, linear system, phytochemistry, practice guideline, process development, quantitative analysis, reproducibility, sensitivity analysis
Como citar
Latin American Journal of Pharmacy, v. 32, n. 1, p. 90-95, 2013.