Comparison of three molecular typing methods to assess genetic diversity for Mycobacterium tuberculosis

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Pitondo-Silva, André
Santos, Adolfo Carlos Barreto [UNESP]
Jolley, Keith A.
Leite, Clarice Queico Fujimura [UNESP]
Darini, Ana Lúcia da Costa

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This study describes the comparison of three methods for genotyping of Mycobacterium tuberculosis, namely MIRU-VNTR (mycobacterial interspersed repetitive units-variable number of tandem repeats), spoligotyping and, for the first time, MLST (Multilocus Sequence Typing). In order to evaluate the discriminatory power of these methods, a total of 44 M. tuberculosis isolates obtained from sputum specimens of patients from Brazil were genotyped. Among the three methods, MLST showed the lowest discriminatory power compared to the other two techniques. MIRU-VNTR showed better discriminatory power when compared to spoligotyping, however, the combination of both methods provides the greatest level of discrimination and therefore this combination is the most useful genotyping tool to be applied to M. tuberculosis isolates. © 2013 Elsevier B.V.



M. tuberculosis, MIRU-VNTR, MLST, Spoligotyping, adult, bacterial genome, bacterial strain, bacterium identification, bacterium isolate, Brazil, cladistics, controlled study, gene cluster, genetic variability, human, intermethod comparison, molecular model, molecular typing, multilocus sequence typing, Mycobacterium tuberculosis, nonhuman, nucleotide sequence, priority journal, sequence analysis, variable number of tandem repeat, Adult, Aged, Aged, 80 and over, Female, Genetic Variation, Humans, Male, Middle Aged, Molecular Epidemiology, Molecular Typing, Sensitivity and Specificity, Sputum, Tuberculosis, Pulmonary, Young Adult

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Journal of Microbiological Methods, v. 93, n. 1, p. 42-48, 2013.