Monitoramento da qualidade higiênico-sanitária no processamento do ''leite'' de soja na UNISOJA, Araraquara, SP
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Data
2003-12-01
Autores
Benedetti, Ana Claudia Elias Pião [UNESP]
Falcão, Deise Pasetto [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Sociedade Brasileira de Ciência e Tecnologia de Alimentos
Resumo
O monitoramento da qualidade higiênico-sanitária do processamento do leite de soja, na UNISOJA, foi realizado através da pesquisa de E. coli e/ou a determinação do NMP de coliformes totais, coliformes fecais (45ºC) e E. coli em dez amostragens consecutivas (236 amostras), coletadas e analisadas em diferentes etapas do processamento. O isolamento de E. coli foi realizado em Ágar EMB, seguido de confirmação bioquímica. Na determinação do NMP foi utilizada a técnica dos tubos múltiplos, que foi calculado utilizando-se a tabela de Hoskins, exceto para o lavado das mãos, cujos resultados foram em termos de presença/ausência. E. coli não foi isolada, quer através da semeadura direta ou a partir dos tubos positivos de Caldo EC. Coliformes totais e/ou coliformes fecais (45ºC) estavam presentes em 42% do total das amostras examinadas, mas a contaminação do produto final, quanto a coliformes fecais, encontrava-se abaixo da determinada pela legislação brasileira (RDC Nº 12, de 02 de janeiro de 2001), portanto dentro dos padrões microbiológicos vigentes no país.
A control of the sanitary-hygienic conditions during the soymilk production was performed at UNISOJA, through the isolation of E. coli and/or the determination of the MPN of total coliform, fecal coliform (45º) and E. coli . In ten occasions, 236 samples were collected and analyzed at different processing stages. The isolation of E. coli was performed using EMB Agar with biochemical confirmation. For the MPN it was used the multiple tube test . The MPN were calculated using the Hoskins table. The results for the samples of washing water hands were given in terms of presence/absence. E. coli was not isolated and its NMP was not determined in any of the analysis realized. Total coliform and/or fecal coliform (45ºC) were present in 42% of the examined samples, but the contamination of the final product, regarding to fecal coliform was inferior to that established by the Brazilian Legislation (RDC. Nº 12, 01/02/2001), therefore in accordance with the microbiological patterns for soymilk in vigor in Brazil .
A control of the sanitary-hygienic conditions during the soymilk production was performed at UNISOJA, through the isolation of E. coli and/or the determination of the MPN of total coliform, fecal coliform (45º) and E. coli . In ten occasions, 236 samples were collected and analyzed at different processing stages. The isolation of E. coli was performed using EMB Agar with biochemical confirmation. For the MPN it was used the multiple tube test . The MPN were calculated using the Hoskins table. The results for the samples of washing water hands were given in terms of presence/absence. E. coli was not isolated and its NMP was not determined in any of the analysis realized. Total coliform and/or fecal coliform (45ºC) were present in 42% of the examined samples, but the contamination of the final product, regarding to fecal coliform was inferior to that established by the Brazilian Legislation (RDC. Nº 12, 01/02/2001), therefore in accordance with the microbiological patterns for soymilk in vigor in Brazil .
Descrição
Palavras-chave
leite de soja, coliformes totais, coliformes fecais (45ºC), E. coli, monitoramento higiênico-sanitário, soymilk, total coliform, fecal coliform (45ºC), E. coli, hygienic monitoring
Como citar
Food Science and Technology (Campinas). Sociedade Brasileira de Ciência e Tecnologia de Alimentos, v. 23, p. 200-205, 2003.